PMID: 7036751Jan 1, 1982Paper

Intracellular pH mediates action of insulin on glycolysis in frog skeletal muscle

The American Journal of Physiology
M L FidelmanR D Moore

Abstract

In a glucose-free bicarbonate Ringer (5% CO2 in N2), insulin increased intracellular pH (pHi), as determined by [14C]dimethadione, by 0.12 +/- 0.02 and stimulated glycolysis, as monitored by anaerobic lactate production, by 42.9 +/- 3.5% in paired frog sartorius muscles. The effect of insulin on glycolysis was shown to vary approximately linearly with log [Na+]0, being converted in 0.12 mM Na+ Ringer to a 51.5 +/- 8.4% inhibition of glycolysis. As the Na+ free-energy gradient was varied by decreasing [Na+]0 from 104 to 6.8 mM, the changes in glycolytic flux produced by insulin consistently paralleled the changes in pHi produced by the hormone. The relationship between the change in pHi and percent change in glycolytic flux was the same regardless of whether the effects were produced by insulin or by changing CO2. When glycolysis was either stimulated or inhibited, intracellular levels of fructose 6-phosphate varied inversely with glycolytic flux. This indicates that the effect on glycolysis of either insulin or changes in CO2 is due to a change in the activity of phosphofructokinase. The results support the model that the acute effect of insulin on glycolysis is mediated by a change in pHi, consequent to activation by insulin o...Continue Reading

References

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Citations

Jan 1, 1989·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M J Fisher, P F Dillon
Nov 26, 2002·Annals of Neurology·Nicola J RobertsonA David Edwards
May 1, 2010·Nanotechnology·Yen-Chi ChenHiroshi Mizukami
Aug 22, 2012·Journal of Vascular Research·Ebbe Boedtkjer, Christian Aalkjaer
Jan 1, 1989·Annals of the New York Academy of Sciences·R W Putnam
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Oct 22, 2002·American Journal of Physiology. Endocrinology and Metabolism·Jing YangGeoffrey D Holman
Sep 13, 2018·Current Vascular Pharmacology·Marco A RamírezLuis Sobrevia
Feb 5, 2019·Cancer Metastasis Reviews·Ebbe Boedtkjer
Nov 16, 2019·Annual Review of Physiology·Ebbe Boedtkjer, Stine F Pedersen
Oct 1, 1991·Postgraduate Medical Journal·P A RutherfordR Wilkinson
Jun 11, 1999·The American Journal of Physiology·M ShahM Baum
Jan 1, 1985·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·A MalanF Daull
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Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R G Smith
Jan 1, 1985·The International Journal of Biochemistry·J NordenbergA Novogrodsky

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