Intracellular pH regulation during prolonged hypoxia in rats

Respiration Physiology
N C Gonzalez, R L Clancy


Conscious rats maintained for three weeks at PB 370-380 Torr were studied in a chamber where PIO2 was maintained at 68-70 Torr at ambient barometric pressure (740-750 Torr). Controls were pair-fed rats maintained at ambient barometric pressure and studied at ambient PIO2 for 4 h. Steady-state intracellular pH (pHi) of left and right ventricle, and of tibialis anterior, quadriceps and diaphragm was determined from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO). Apparent non-bicarbonate buffer value (beta app) was calculated as the ratio of the change in HCO3- concentration to the change in pH elicited by the increase in PCO2. beta app of plasma, tibialis anterior, quadriceps and diaphragm was approximately 2, 3, 6 and 12 times higher, respectively, in hypoxic than in normoxic rats. Neither left nor right ventricular beta app was significantly changed by prolonged hypoxia. In the hypoxic animals, bilateral nephrectomy abolished the increase in beta app of plasma, tibialis anterior and quadriceps, and moderated the increase in beta app of diaphragm. No significant effect of nephrectomy was observed in beta app of either left or right ventricle. The results indicate that in the skeletal muscles studied under conditions...Continue Reading


Sep 29, 1978·Pflügers Archiv : European journal of physiology·D Cechetto, G W Mainwood
May 1, 1986·Respiration Physiology·N C Gonzalez, R L Clancy
Nov 1, 1973·Respiration Physiology·Y L LaiE B Brown


Sep 1, 1986·Respiration Physiology·G WidenerN C Gonzalez
Feb 6, 2004·Clinical and Experimental Pharmacology & Physiology·Yumi OkitsuTakashi Nishino

Related Concepts

Anion Gap
Carbonic Acid Ions
Carbon Dioxide
Hydrogen-Ion Concentration
August Rats

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

Related Papers

American Journal of Respiratory and Critical Care Medicine
H E GreenbergM ] Kuperferman M [corrected to Kupferman
© 2021 Meta ULC. All rights reserved