Intracellular processing of 125I-epidermal growth factor in rat embryo fibroblasts

Journal of Cellular Biochemistry
B E MagunH N Wagner

Abstract

The intracellular fate of endocytosed 125I-epidermal growth factor was examined in Rat-1 fibroblasts. Cells were pulse-labeled for 5 min in 125I-EGF and chased for 3 hr with an excess of unlabeled EGF. At various times after application of the cold chase, cells were harvested and processed for isopycnic gradient centrifugation on Percoll gradients. Within the period of the 125I-EGF pulse, about 50% of the 125I activity appeared in an organelle containing peak in the gradients. By 20 min after application of the cold chase, 125I activity in the organelle peak began to decrease, and the decrease continued over the next few hours. The 125I activity which exited from its organelle-associated location appeared to be present in the cytosol and was apparently not confined within organelles. Lysosomotropic amines inhibited the egress of 125I activity from the organelle compartment. The 125I activity from both organelle and nonorganelle compartments reacted as completely as authentic 125I-EGF with anti-EGF antibodies and was similar in size to authentic 125I-EGF. Little or no intracellular low molecular weight 125I-containing compounds were detected, although they accumulated in the culture medium. Analytical isoelectric focusing reveal...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S Ohkuma, B Poole
Sep 1, 1977·The Histochemical Journal·A B Novikoff, P M Novikoff
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A McKannaS Cohen
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J SchlessingerI Pastan
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H HaiglerS Cohen
May 1, 1976·Experimental Cell Research·A Lindgren, B Westermark
Oct 30, 1981·Science·I H Pastan, M C Willingham
Jan 1, 1981·Annual Review of Physiology·I H Pastan, M C Willingham
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A C KingP Cuatrecasas
Sep 1, 1981·Journal of Cellular Physiology·L M MatrisianB E Magun
Sep 15, 1982·Analytical Biochemistry·L M MatrisianB E Magun

❮ Previous
Next ❯

Citations

Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H SunadaC L MacLeod
Jun 1, 1988·Journal of Cellular Biochemistry·K N Fedde, W S Sly

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.