Jan 1, 1976

Intracellular protein breakdown. VII. Cathepsin L and H; two new proteinases from rat liver lysosomes

Acta biologica et medica Germanica
H KirschkeU Broghammer

Abstract

Some properties (molecular weight, pI, temperature stability, action of selected inhibitors, substrate specificity and pH-activity dependence) of two not yet known cathepsins from rat liver lysosomes are compared with the properties of the known cathepsin B1. Cathepsin L is a thiolproteinase, has a molecular weight of 23--24000 and a pI of 5,8--6,1. By disc electrophoresis and isoelectric focusing there appear several protein bands which all have enzymatic activity. Leupeptin behaves as a strong inhibitor. The pH-optimum for digestion of proteins is close to 5,0. Cathepsin L does not hydrolyse esters and splits synthetic low molecular substrates only to a low degree. Cathepsin L stored in presence of glutathion and EDTA in liquid nitrogen kept its activity for some months. Cathepsin H is an aminopeptidase as well as an endopeptidase. An enzyme with these bifunctional properties was detected up to now only in E. coli but not in animal cells. Cathepsin H is a thiol-enzyme with a molecular weight of 28000 and a pI of 7,1. Strong inhibitors are leucyl-chlormethan and SH-blocking substances. Leupeptin shows only a weak inhibitory effect to this enzyme compared to its action on cathepsins L and B1. The pH-optimum for hydrolysis of al...Continue Reading

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Mentioned in this Paper

Amino acid derivative
Protein Degradation, Regulatory
Protoplasm
Peptide Hydrolases
Cathepsin H
Cathepsins B
Aminopeptidase
Endopeptidases
Sulfhydryl Compounds
Electrophoresis, Disc

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