Intracellular protein delivery and gene transfection by electroporation using a microneedle electrode array.

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Seong-O ChoiMark G Allen

Abstract

The impact of many biopharmaceuticals, including protein- and gene-based therapies, has been limited by the need for better methods of delivery into cells within tissues. Here, intracellular delivery of molecules and transfection with plasmid DNA by electroporation is presented using a novel microneedle electrode array designed for the targeted treatment of skin and other tissue surfaces. The microneedle array is molded out of polylactic acid. Electrodes and circuitry required for electroporation are applied to the microneedle array surface by a new metal-transfer micromolding method. The microneedle array maintains mechanical integrity after insertion into pig cadaver skin and is able to electroporate human prostate cancer cells in vitro. Quantitative measurements show that increasing electroporation pulse voltage increases uptake efficiency of calcein and bovine serum albumin, whereas increasing pulse length has lesser effects over the range studied. Uptake of molecules by up to 50% of cells and transfection of 12% of cells with a gene for green fluorescent protein is demonstrated at high cell viability. It is concluded that the microneedle electrode array is able to electroporate cells, resulting in intracellular uptake of m...Continue Reading

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Citations

Jul 17, 2013·Advanced Drug Delivery Reviews·Eric Nuxoll
May 12, 2012·Advanced Drug Delivery Reviews·Yeu-Chun KimMark R Prausnitz
Aug 19, 2014·Bioconjugate Chemistry·Ailing FuVincent M Rotello
May 23, 2015·Scientific Reports·Gregory Lee SzetoDarrell J Irvine
Jan 23, 2014·Advanced Materials·Li YanXianfeng Chen
Jun 26, 2015·ACS Nano·Sahishnu PatelKi-Bum Lee
Oct 19, 2016·Lab on a Chip·Lingqian ChangLy James Lee
Mar 15, 2018·Chemical Society Reviews·Federica ScalettiVincent M Rotello
Nov 21, 2020·Pharmaceutics·Aravindram Attiguppe SeetharamAfrasim Moin
Jan 4, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Suman PahalPraveen Kumar Vemula
Dec 29, 2020·Chemistry : a European Journal·Josep GarciaMacarena Sánchez-Navarro
May 1, 2021·Pharmaceuticals·Sara BusattoJoy Wolfram
May 14, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Defu ZhiRyan F Donnelly
Mar 10, 2020·ACS Applied Materials & Interfaces·Shuting PanVincent M Rotello
Jun 11, 2020·Nano Letters·Yuan WangPeng Shi
Oct 2, 2020·ACS Nano·Xingwu ZhouAli Khademhosseini
Jun 20, 2020·ACS Applied Materials & Interfaces·Yangcui QuHong Chen
Oct 22, 2021·Proceedings of the National Academy of Sciences of the United States of America·Dengning XiaMark R Prausnitz

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