PMID: 3763380Sep 1, 1986Paper

Intracellular recordings from isolated rabbit retinal Müller (glial) cells

Pflügers Archiv : European journal of physiology
A Reichenbach, W Eberhardt

Abstract

Müller (glial) cells were isolated from rabbit retinae by papaine and mechanical dissociation. The cells were fixed on a gelatine-covered glass slide by means of concanavalin A, and the slide was mounted in a perfusion chamber under a light microscope with modified optics. Besides the recording microelectrode, two other micropipettes could be adjusted with their tips near the cell. These micropipettes were used for application of test solutions into the environment of the cells. On application of high K+ solutions, the cell depolarized strongly but during prolonged application there was a marked repolarization. After the end of high K+ application the cells showed a hyperpolarization which was enhanced in both amplitude and duration with prolongation of the K+ exposure. Both repolarization and afterhyperpolarization disappeared under ouabain. Ouabain application itself caused a small reversible depolarization. Na+ free solution caused hyperpolarization. The results suggest the existence of an active membrane pump mechanism in our cells. This pump seems to be electrogenic under our experimental conditions and seems to be activated even in the absence of sodium. The cell membrane is demonstrated to contain a significant Na+ condu...Continue Reading

References

Sep 1, 1978·The Journal of Cell Biology·P V Sarthy, D M Lam
Jan 31, 1977·Brain Research·R F MillerL Proenza
Mar 1, 1977·Journal of Neurophysiology·C J Karowski, L M Proenza
Jan 1, 1975·Annual Review of Physiology·G G Somjen
Mar 5, 1976·Brain Research·G KukesJ De Vellis
May 1, 1985·The Journal of Physiology·J D ConnerP V Sarthy
Sep 23, 1985·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·S BevanJ M Ritchie
Jan 1, 1966·Ergebnisse der Physiologie, biologischen Chemie und experimentellen Pharmakologie·S W Kuffler, J G Nicholls
Mar 1, 1984·Pflügers Archiv : European journal of physiology·H KettenmannH D Lux

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Citations

Sep 1, 1990·Documenta Ophthalmologica. Advances in Ophthalmology·W U Mättig, R Hanitzsch
Jun 1, 1988·Pflügers Archiv : European journal of physiology·B Nilius, A Reichenbach
Mar 31, 1987·Neuroscience Letters·A ReichenbachW Eberhardt
Jan 1, 1996·Acta neurochirurgica·T KamiryoL Steiner
Jan 1, 1989·Critical Reviews in Clinical Laboratory Sciences·M T Haupt
Nov 19, 2020·Journal of Neurophysiology·Boriana K TchernookovaRobert Paul Malchow

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