Intracellular redistribution of interferon-inducible proteins Nmi and IFP 35 in apoptotic cells

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
Jun Chen, L Naumovski

Abstract

As a consequence of the activation of the apoptotic pathway, the subcellular localization of many proteins that modulate cell death is altered. Nmi and IFP 35 are homologous interferon (IFN)-inducible proteins that associate into a 300-400-kDa complex in the cytoplasm. Prior to treatment with IFN, subcellular fractionation reveals that the proteins are found primarily in a soluble cytoplasmic fraction, and immunofluorescence shows a diffuse cytoplasmic distribution. Treatment with IFN results in some Nmi and IFP 35 fractionating with membranes and colocalizing into speckles as detected by immunofluorescence. In IFN-treated apoptotic cells, the speckles dissociate and are replaced by a diffuse cytoplasmic distribution of Nmi and IFP 35. In Jurkat cells, dissociation of speckles could not be separated from other markers of apoptosis, including exposure of phosphatidylserine on the cell surface, caspase cleavage of D4-GDI, and nuclear condensation and fragmentation. The caspase inhibitor, zVAD-fmk, was not able to inhibit the dissociation of speckles in vivo or in a permeabilized cell assay in vitro, suggesting that dissociation of speckles is a caspase-independent process. Nmi and IFP 35 proteins are not caspase substrates, as to...Continue Reading

References

Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y T HsuR J Youle
May 16, 1998·The Journal of Biological Chemistry·P King, S Goodbourn
Oct 22, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·S J LebrunL Naumovski
Jan 16, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A MeyerdierksE C Böttger
Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Nov 24, 1999·The Journal of Biological Chemistry·J MasumotoJ Sagara
Nov 26, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·N D LeeL Naumovski
Jun 13, 2001·The Journal of Cell Biology·A NechushtanR J Youle

❮ Previous
Next ❯

Citations

Nov 12, 2010·Domestic Animal Endocrinology·G SongF W Bazer
Apr 5, 2007·Molecular and Cellular Biochemistry·Keman ZhangYu-Chung Yang
Jun 17, 2020·Genome Biology·Craig H KerrLeonard J Foster
Oct 28, 2020·Cell Biochemistry and Biophysics·Songbin WuHongjian Li
Jan 16, 2010·Journal of Proteome Research·Yanping SongFuchu He

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis