Intracellular regulation of progesterone secretion by the superoxide radical in the rat corpus luteum

Endocrinology
M Sawada, J C Carlson

Abstract

In this study we examined the prospect that the superoxide radical (SOR) is involved in the mechanism by which LH stimulates progesterone secretion in the rat corpus luteum (CL). Treatment of dispersed CL cells with low doses of LH or a SOR-generating system (xanthine-xanthine oxidase) resulted in a significant increase in progesterone release and SOR production. High doses of each treatment were inhibitory. SOR generation also decreased hCG binding. To determine whether SOR may be required for progesterone secretion, dispersed cells were electroporated with antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)] and treated with either low (50 ng) stimulatory or high (20 micrograms) inhibitory doses of LH. At 50 ng LH, insertion of SOD or CAT dose-dependently inhibited progesterone secretion. However, at high doses of LH (20 micrograms), which are associated with high levels of SOR, electroporation of SOD or CAT produced the opposite response. This stimulatory response of SOD or CAT on progesterone release was also dose related. These results indicate that SOR may be involved in the mechanisms that stimulate as well as those that inhibit progesterone release. The effect on progesterone secretion appears to be dose ...Continue Reading

Citations

Sep 3, 2005·Reproductive Biology and Endocrinology : RB&E·Junichi FujiiFutoshi Okada
Aug 8, 2001·Reproductive Toxicology·J A NykampJ C Carlson
Oct 16, 2012·Reproductive Biomedicine Online·Kaïs H Al-GuboryNorihiro Sugino
Mar 23, 2011·Regulatory Peptides·Laura BaioniFrancesca Grasselli
Feb 23, 2010·Regulatory Peptides·Arash KheradmandMahdi Birjandi
Sep 24, 2015·General and Comparative Endocrinology·Manika Kala, Manish Nivsarkar
Nov 21, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Jitender K Bhardwaj, Priyanka Saraf
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Sep 22, 2020·Systems Biology in Reproductive Medicine·Elif Erdem GuzelIbrahim Enver Ozan
Nov 18, 2017·Oxidative Medicine and Cellular Longevity·Shan WangXinghui Liu
Jul 16, 2020·Journal of the Endocrine Society·Bartira Marques PizarroTania Maria Ortiga-Carvalho
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Apr 25, 2021·Ageing Research Reviews·F Timóteo-FerreiraE Silva
Aug 26, 2021·Human Reproduction Update·Alexa BishopGuy S Whitley

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