Intracellular ROS generated in chikungunya patients with persisting polyarthralgia can be reduced by Tinospora cordifolia leaf extract

Virusdisease
Nilotpal BanerjeeSumi Mukhopadhyay

Abstract

Chikungunya (CHIK) is an arboviral infection having huge global burden affecting the life style of the patient badly due to debilitating polyarthralgia. This study aims to evaluate intracellular reactive oxygen species (ROS) in peripheral blood of patients suffering with persisting polyarthralgia post CHIK infection and the potential of Tinospora cordifolia leaf extract in scavenging those free radicals in peripheral blood mononuclear cells (PBMC) of the patient. Peripheral blood was collected from written informed consented patients and intracellular ROS was measured in PBMC of patients suffering with persisting polyarthralgia 3 months post CHIK infection followed by the study of free radical scavenging by T. cordifolia leaf extract in those cells through flow cytometry. Control population comprising healthy donors were also included in the study. As compared to healthy subjects, twofold higher Intracellular ROS (17.89 ± 1.007 vs. 37.96 ± 1.510, P < 0.0001) was found in patient PBMC. Ex-vivo treatment of those PBMC with ethanolic extract of T. cordifolia leaf (1 μg/mL) decreased intracellular ROS significantly by twofold (P < 0.0001). This study reports that CHIK infection produces high level of intracellular ROS in the patien...Continue Reading

References

Feb 23, 2007·The New England Journal of Medicine·Rémi N CharrelDidier Raoult
Jan 15, 2010·Methods in Molecular Biology·Evgeniy Eruslanov, Sergei Kusmartsev
Feb 1, 2012·Journal of Food Science and Technology·M SaralaR P Singh
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Sep 3, 2013·Antioxidants & Redox Signaling·Manish MittalAsrar B Malik
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Dec 3, 2015·Transactions of the Royal Society of Tropical Medicine and Hygiene·Jaime A Cardona-OspinaAlfonso J Rodríguez-Morales
Mar 1, 2016·Virusdisease·Nilotpal Banerjee, Sumi Mukhopadhyay

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Citations

Sep 8, 2021·Environmental Science and Pollution Research International·Rajeshwari SinghDevesh Tewari

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Methods Mentioned

BETA
flow cytometry
ELISA
PCR
Fluorescence

Software Mentioned

Makonde
GraphPad Prism

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