PMID: 9177198Jun 10, 1997Paper

Intracellular structures of normal and aberrant Plasmodium falciparum malaria parasites imaged by soft x-ray microscopy

Proceedings of the National Academy of Sciences of the United States of America
C MagowanW Meyer-Ilse

Abstract

Soft x-ray microscopy is a novel approach for investigation of intracellular organisms and subcellular structures with high spatial resolution. We used x-ray microscopy to investigate structural development of Plasmodium falciparum malaria parasites in normal and genetically abnormal erythrocytes and in infected erythrocytes treated with cysteine protease inhibitors. Investigations in normal red blood cells enabled us to recognize anomalies in parasite structures resulting from growth under unfavorable conditions. X-ray microscopy facilitated detection of newly elaborated structures in the cytosol of fixed, unstained, intact erythrocytes, redistribution of mass (carbon) in infected erythrocytes, and aberrant parasite morphology. In cysteine protease inhibitor-treated, infected erythrocytes, high concentrations of material were detected in abnormal digestive vacuoles and aggregated at the parasite plasma membrane. We have demonstrated that an abnormal host erythrocyte skeleton affects structural development of parasites and that this aberrant development can be detected in the following generation when parasites from protein 4.1-deficient red blood cells infect normal erythrocytes. This work extends our current understanding of ...Continue Reading

References

Nov 1, 1978·The Journal of protozoology·S G LangrethW Trager
Feb 1, 1978·Annals of Tropical Medicine and Parasitology·G PasvolJ Brown
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A Kilejian
Nov 1, 1991·The Journal of Clinical Investigation·P J RosenthalD Rasnick
Feb 1, 1991·Molecular and Biochemical Parasitology·A KilejianY F Yang
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D E GoldbergG B Henderson
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S SchulmanR S Schwartz
Sep 1, 1987·Molecular and Biochemical Parasitology·D W TaylorR J Howard
Nov 1, 1988·The Journal of Clinical Investigation·P J RosenthalJ H Leech
Dec 1, 1986·Experimental Parasitology·A R DluzewskiW B Gratzer
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R F AndersA Edwards
Feb 1, 1995·Quarterly Reviews of Biophysics·J KirzM Howells
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·J H WilliamsW Trager
Jul 11, 1996·The New England Journal of Medicine·S L Hoffman
May 1, 1997·Transactions of the Royal Society of Tropical Medicine and Hygiene·S L CranmerB M Cooke
Mar 1, 1993·Parasitology Today·B C Elford, D J Ferguson

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Citations

Dec 10, 1998·International Journal for Parasitology·M Foley, L Tilley
Mar 24, 1999·Trends in Cell Biology·C Jacobsen
Nov 5, 2004·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Felix A RuizRoberto Docampo
Aug 12, 1998·Nature Structural Biology·C Jacobsen, J Kirz
Sep 3, 2013·Malaria Journal·Yulia M SerebrennikovaJennifer M Smith
Jul 2, 2010·The Review of Scientific Instruments·Jun LimChung Ki Hong
Oct 20, 2012·Journal of Structural Biology·Allon WeinerMichael Elbaum
Sep 21, 2011·Trends in Biotechnology·Sangyeon ChoYongKeun Park
Sep 10, 2010·Journal of Structural Biology·Eric HanssenLeann Tilley
Feb 24, 2010·Journal of Biomechanics·Yohsuke ImaiTakami Yamaguchi
Sep 1, 2007·Experimental Parasitology·Kildare MirandaSilvia N J Moreno
Apr 29, 2005·Journal of Microscopy·S Jearanaikoon, J V Abraham-Peskir
Aug 2, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Garth J WilliamsKeith A Nugent
Apr 17, 2012·Microscopy Research and Technique·Ting ZhengYangchao Tian
May 26, 2006·Micron : the International Research and Review Journal for Microscopy·Michael G AndersonMichael Grunze
Mar 3, 2006·Physics in Medicine and Biology·Kyong Woo KimKwon-Ha Yoon
Aug 16, 2002·Journal of Microscopy·S C MayoA W Stevenson
Jul 3, 2015·Journal of Synchrotron Radiation·Shengxiang WangZiyu Wu
Apr 7, 2019·Biochemical Society Transactions·Venera WeinhardtCarolyn Larabell

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