PMID: 9632312Jun 19, 1998Paper

Intracellular sulfatide expression in a subpopulation of astrocytes in primary cultures

Journal of Neuroscience Research
Z BerntsonP Fredman

Abstract

A highly specific antibody against sulfatide, a myelin-associated glycolipid, has been investigated using indirect double immunocytochemistry in rat primary astroglial cultures from cerebral cortex. Sulfatide was expressed in a selected subpopulation of astrocytes (2-3%) and was found to be exclusively located intracellularly. The sulfatide-positive cells appeared in two different morphologies: flat and stellate. Immunolabeling of the astroglial cultures showed that sulfatide always co-existed with GFAP or S-100, and in some cells with GD3 (flat 90% and stellate 50%) or A2B5 (1%) antibody. The sulfatide-positive cells did not bind the O1 antibody, which is used as a marker for oligodendrocytes. Glial cultures from other regions and mixed cultures, with both neurons and glial cells, were examined and showed similar results. Biochemical analysis by TLC-ELISA verified the presence of sulfatide in the astroglial culture and showed decreasing amounts of sulfatide with days in vitro; 0.05 nmol/mg protein at day 10 and 0.01 nmol/mg protein at day 17. This analysis also showed that neither sulpholactosylceramide nor seminolipid was present, each of which also has affinity for the sulfatide antibody. This selective and intracellular exp...Continue Reading

Citations

Dec 4, 2009·PloS One·Karen E DrahosDaniel G S Capelluto
May 24, 2012·Journal of Lipid Research·Tadanobu Takahashi, Takashi Suzuki
Nov 7, 2015·Neurochemical Research·Sara GrassiAlessandro Prinetti
May 15, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Elizabeth SuescaJohn M González
Jun 20, 2003·Journal of Neuroscience Research·Diane M Studzinski, Joyce A Benjamins
May 10, 2008·Molecular Neurobiology·Matthias Eckhardt
Apr 21, 2006·The FEBS Journal·Giorgis IsaacJan-Eric Månsson
Jul 26, 2011·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hitoshi KajigayaHiroko Baba
Jul 23, 2021·Proceedings of the National Academy of Sciences of the United States of America·Lijing SuBruce Beutler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

© 2022 Meta ULC. All rights reserved