Intracellular survival and replication of Erysipelothrix rhusiopathiae within murine macrophages: failure of induction of the oxidative burst of macrophages.

Infection and Immunity
Y ShimojiY Mori

Abstract

We investigated the ability of a virulent wild-type parent strain and acapsular avirulent transposon mutants to enter and survive intracellularly within murine peritoneal macrophages. In the presence of normal or immune serum, the parent and mutant strains were both ingested; however, the number of ingested bacteria was three- to fourfold greater in the case of mutant strains than in the case of the parent strain. The parent strain, but not the mutant strains, survived and replicated intracellularly when ingested in the presence of normal serum, whereas both the parent and the mutant strains were readily killed when ingested in the presence of immune serum. To further investigate the mechanism by which the parent strain can survive and replicate within macrophages, we studied the oxidative burst response of macrophages to these strains by measuring chemiluminescence and intracellular reduction of Nitro Blue Tetrazolium dye. Challenge exposure of macrophages with either the parent strain preopsonized with immune serum or the mutant strains preopsonized with normal or immune serum induced a strong oxidative burst, whereas the level was very low when the parent strain was preopsonized with normal serum. Phagocytosis of either the ...Continue Reading

References

Nov 1, 1987·The Journal of Experimental Medicine·N R Payne, M A Horwitz
Nov 1, 1985·Infection and Immunity·C J Czuprynski, H L Hamilton
Dec 1, 1983·The Journal of Experimental Medicine·S D Wright, S C Silverstein
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Jul 1, 1993·Infection and Immunity·M K HondalusD M Mosser

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Citations

Mar 27, 2002·The Journal of Veterinary Medical Science·Yoshihiro ShimojiYuichi Yokomizo
Dec 17, 2009·Applied and Environmental Microbiology·Laura L PresleyJames Borneman
Aug 26, 1998·FEMS Immunology and Medical Microbiology·M A SeguraM Gottschalk
Apr 23, 2013·Veterinary Immunology and Immunopathology·Tomoyuki HaradaYoshihiro Shimoji

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