Intracellular survival of Staphylococcus aureus during persistent infection in the insect Tenebrio molitor

Developmental and Comparative Immunology
John E McGonigleJens Rolff

Abstract

Survival of bacteria within host cells and tissues presents a challenge to the immune systems of higher organisms. Escape from phagocytic immune cells compounds this issue, as immune cells become potential vehicles for pathogen dissemination. However, the duration of persistence within phagocytes and its contribution to pathogen load has yet to be determined. We investigate the immunological significance of intracellular persistence within the insect model Tenebrio molitor, assessing the extent, duration and location of bacterial recovery during a persistent infection. Relative abundance of Staphylococcus aureus in both intracellular and extracellular fractions was determined over 21 days, and live S. aureus were successfully recovered from both the hemolymph and within phagocytic immune cells across the entire time course. The proportion of bacteria recovered from within phagocytes also increased over time. Our results show that to accurately estimate pathogen load it is vital to account for bacteria persisting within immune cells.

References

Mar 1, 1996·The Journal of Infectious Diseases·O VesgaR A Proctor
Oct 8, 1999·The Journal of Antimicrobial Chemotherapy·W O ChungM C Roberts
Jul 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·P J DabornR H Ffrench-Constant
Nov 5, 2003·The Journal of Clinical Investigation·Morten Hentzer, Michael Givskov
Sep 17, 2004·Nature Reviews. Microbiology·Denise M MonackStanley Falkow
Feb 1, 2005·The Journal of Antimicrobial Chemotherapy·M B KerrnK A Krogfelt
Aug 19, 2005·The Journal of Infectious Diseases·Sophie ClementJean-Silvain Lacroix
Jan 9, 2007·Current Opinion in Microbiology·Alexandra F Freeman, Steven M Holland
Jun 3, 2008·Journal of Insect Physiology·Eleanor R HaineJens Rolff
Oct 18, 2008·Nature Reviews. Immunology·David S Schneider, Janelle S Ayres
Nov 22, 2008·Science·Eleanor R HaineJens Rolff
Sep 15, 2009·Cell Host & Microbe·Naoaki ShinzawaHirotaka Kanuka
Oct 12, 2010·Journal of Invertebrate Pathology·Isabella VlisidouNick R Waterfield
Mar 1, 2012·Science·Ruslan MedzhitovMiguel P Soares
Jun 23, 2012·Open Biology·Ilias Kounatidis, Petros Ligoxygakis
Apr 25, 2015·Molecular Biology and Evolution·William J Palmer, Francis M Jiggins

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Citations

May 11, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Olga MakarovaJens Rolff
Mar 21, 2020·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Maria Hassan KianiGul Shahnaz
May 10, 2017·Ecology and Evolution·Rebeca B RosengausWendy Smith

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