Intracellular trafficking of glycosylphosphatidylinositol (GPI)-anchored proteins and free GPIs in Leishmania mexicana

The Biochemical Journal
J E RaltonMalcolm J McConville

Abstract

Free glycosylphosphatidylinositols (GPIs) are an important class of membrane lipids in many pathogenic protozoa. In this study, we have investigated the subcellular distribution and intracellular trafficking of an abundant class of free GPIs [termed glycosylinositolphospholipids (GIPLs)] in Leishmania mexicana promastigotes. The intracellular transport of the GIPLs and the major GPI-anchored glycoprotein gp63 was measured by following the incorporation of these molecules into sphingolipid-rich, detergent-resistant membranes (DRMs) in the plasma membrane. In metabolic-labelling experiments, mature GIPLs and gp63 were transported to DRMs in the plasma membrane with a t(1/2) of 70 and 40 min, respectively. Probably, GIPL transport to the DRMs involves a vesicular mechanism, as transport of both the GIPLs and gp63 was inhibited similarly at 10 degrees C. All GIPL intermediates were quantitatively recovered in Triton X-100-soluble membranes and were largely orientated on the cytoplasmic face of the endoplasmic reticulum, as shown by their sensitivity to exogenous phosphatidylinositol-specific phospho-lipase C. On the contrary, a significant proportion of the mature GIPLs ( approximately 50% of iM4) were accessible to membrane-imperm...Continue Reading

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Citations

Oct 24, 2002·International Journal for Parasitology·Ross F Waller, Malcolm J McConville
Dec 7, 2002·Molecular and Biochemical Parasitology·Thomas Naderer, Malcolm J McConville
Oct 18, 2003·Molecular and Biochemical Parasitology·Chaoqun YaoMary E Wilson
Jun 19, 2012·The Journal of Biological Chemistry·Gaspar E CánepaCarlos A Buscaglia
Apr 11, 2003·Molecular Biology of the Cell·Erik VassellaIsabel Roditi
Sep 10, 2011·Cerebral Cortex·Evelyn ErdmannDirk Dietrich
May 1, 2013·Infection and Immunity·Chaoqun YaoMary E Wilson
Dec 22, 2005·Infection and Immunity·Lena J HeungMaurizio Del Poeta
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Malcolm J McConvilleRohan D Teasdale
Sep 24, 2011·Biochemistry Research International·Hayder Z AliPaul W Denny
Mar 10, 2007·Parasitology International·Wanderley de Souza
Jul 17, 2004·Molecular Microbiology·Paul W Denny, Deborah F Smith
Aug 14, 2008·Molecular Microbiology·Shaheen S SutterwalaJames D Bangs
Apr 28, 2004·Molecular and Biochemical Parasitology·Chaoqun YaoMary E Wilson
Jul 25, 2006·Biochimica Et Biophysica Acta·Martine Pittet, Andreas Conzelmann
Feb 20, 2007·Molecular and Biochemical Parasitology·Dayana Rodriguez-ContrerasScott M Landfear
Aug 7, 2003·The Journal of Biological Chemistry·Julie E RaltonMalcolm J McConville
May 19, 2006·The Journal of Biological Chemistry·Dayana Rodríguez-Contreras, Scott M Landfear

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