Intracellular trafficking of recycling apolipoprotein E in Chinese hamster ovary cells.

Journal of Lipid Research
Nicole A BraunLarry L Swift

Abstract

We have investigated apolipoprotein E (apoE) recycling in Chinese hamster ovary (CHO) cells, a peripheral cell that does not produce lipoproteins or express apoE. Using a pulse-chase protocol in which cells were pulsed with 125I-apoE-VLDL and chased for different periods, approximately 30% of the apoE internalized during the pulse was resecreted within a 4 h chase in a relatively lipid-free state. The addition of lysosomotropic agents or brefeldin A had no effect on apoE recycling. Unlike previous results with hepatocytes and macrophages, neither apoA-I nor upregulation of ABCA1 stimulated apoE recycling. However, cyclodextrin, which extracts cholesterol from plasma membrane lipid rafts, increased recycling. Confocal studies revealed that apoE, internalized during a 1 h pulse, colocalizes with early endosomal antigen-1, Rab5, Rab11a, and lysobisphosphatidic acid but not with lysosomal-associated membrane protein-1. Colocalization of apoE and Rab11a persisted even after cells had been chased for 1 h, suggesting a pool of apoE within the endosomal recycling compartment (ERC). Our data suggest that apoE recycling in CHO cells is linked to cellular cholesterol removal via the ERC and phospholipid-containing acceptors in a pathway a...Continue Reading

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Citations

Jun 28, 2011·Journal of Lipid Research·Jianjia FanCheryl L Wellington
Apr 30, 2016·Journal of Alzheimer's Disease : JAD·Michelle PerkinsJon Valla
Apr 5, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Maaike KockxLeonard Kritharides
Mar 8, 2007·The Journal of Biological Chemistry·Daping FanSergio Fazio
Jun 27, 2020·Frontiers in Aging Neuroscience·Hussein N Yassine, Caleb E Finch
Jun 3, 2021·International Journal of Molecular Sciences·Meewhi Kim, Ilya Bezprozvanny

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