Intracellular trafficking of VP22 in bovine herpesvirus-1 infected cells

Virology
Vladislav A LobanovSylvia van Drunen Littel-van den Hurk

Abstract

The intracellular trafficking of different VP22-enhanced yellow fluorescent protein (EYFP) fusion proteins expressed by bovine herpesvirus-1 (BHV-1) recombinants was examined by live-cell imaging. Our results demonstrate that (i) the fusion of EYFP to the C terminus of VP22 does not alter the trafficking of the protein in infected cells, (ii) VP22 expressed during BHV-1 infection translocates to the nucleus through three different pathways, namely early mitosis-dependent nuclear translocation, late massive nuclear translocation that follows a prolonged cytoplasmic stage of the protein in non-mitotic cells, and accumulation of a small subset of VP22 in discrete dot-like nuclear domains during its early cytoplasmic stage, (iii) the addition of the SV40 large-T-antigen nuclear localization signal (NLS) to VP22-EYFP abrogates its early cytoplasmic stage, and (iv) the VP22 (131)PRPR(134) NLS is not required for the late massive nuclear translocation of the protein, but this motif is essential for the targeting of VP22 to discrete dot-like nuclear domains during the early cytoplasmic stage. These results show that the amount of VP22 in the nucleus is precisely regulated at different stages of BHV-1 infection and suggest that the earl...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F C Purves, B Roizman
Jan 10, 1995·Virology·S van Drunen Littel-van den HurkP Tijssen
Mar 21, 2000·Biochimica Et Biophysica Acta·J R Sellers
Jan 11, 2001·Journal of Virology·M P ShermanW C Greene
Jun 27, 2001·The Journal of Cell Biology·M VecchiP P Di Fiore
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·I G Macara
Mar 9, 2002·Journal of Virology·Hans van LeeuwenPeter O'Hare
Jun 27, 2003·Trends in Biochemical Sciences·Joanna Wysocka, Winship Herr
Dec 23, 2004·Journal of Virology·Peter WildPaul Walther
Feb 12, 2005·The Journal of Biological Chemistry·Jun ZhuGary A Splitter
Sep 6, 2005·Journal of Virology·Chunfu ZhengSylvia van Drunen Littel-van den Hurk
Sep 15, 2005·Journal of Virology·G MouzakitisP O'Hare
Dec 28, 2005·Journal of Virology·Janneke VerhagenGillian Elliott
Jan 18, 2006·Journal of Virology·Thomas C Mettenleiter, Tony Minson
Oct 27, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hong JiangJuan Fueyo
Oct 16, 2007·Biochimica Et Biophysica Acta·Gualtiero AlvisiDavid A Jans
Nov 15, 2007·Nature Reviews. Molecular Cell Biology·Ruth Geiss-Friedlander, Frauke Melchior
Jun 20, 2008·Journal of Virology·Helmut Hofemeister, Peter O'Hare

❮ Previous
Next ❯

Citations

Sep 7, 2014·Virus Research·Ayaka OkadaHideto Fukushi
Nov 28, 2012·Journal of Cancer Research and Clinical Oncology·Guishan JinFusheng Liu
Feb 19, 2016·Journal of Virology·Sharmin AfrozSylvia van Drunen Littel-van den Hurk
Aug 28, 2020·Frontiers in Microbiology·Liping WuXiaoyue Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.