Intracellular transcription of G-rich DNAs induces formation of G-loops, novel structures containing G4 DNA

Genes & Development
Michelle L DuquetteNancy Maizels

Abstract

We show that intracellular transcription of G-rich regions produces novel DNA structures, visible by electron microscopy as large (150-500 bp) loops. These G-loops are formed cotranscriptionally, and they contain G4 DNA on one strand and a stable RNA/DNA hybrid on the other. G-loop formation requires a G-rich nontemplate strand and reflects the unusual stability of the rG/dC base pair. G-loops and G4 DNA form efficiently within plasmid genomes transcribed in vitro or in Escherichia coli. These results establish that G4 DNA can form in vivo, a finding with implications for stability and maintenance of all G-rich genomic regions.

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M ThomasR W Davis
Jan 1, 1989·Methods in Enzymology·L T Chow, T R Broker
Oct 1, 1987·Human Genetics·N BurokerM Litt
Oct 1, 1987·Annals of Human Genetics·Z WongA J Jeffreys
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·H B Gamper, J E Hearst
Jun 1, 1995·Current Opinion in Structural Biology·D Rhodes, R Giraldo
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Fry, L A Loeb
Feb 11, 1993·Nucleic Acids Research·W DunnickC Gritzmacher
Oct 1, 1995·Trends in Biochemical Sciences·R D Kolodner
Jan 1, 1996·Annual Review of Biochemistry·P Modrich, R Lahue
Apr 4, 1997·The Journal of Biological Chemistry·M N WeitzmannK Usdin
Oct 9, 1998·The Journal of Biological Chemistry·H SunN Maizels
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·C T McMurray
Apr 13, 1999·Nucleic Acids Research·H SunN Maizels
May 21, 1999·The Journal of Biological Chemistry·L A HanakahiN Maizels
Jun 11, 1999·Current Opinion in Structural Biology·D E Gilbert, J Feigon
Apr 27, 2000·Trends in Genetics : TIG·J C Shen, L A Loeb
Oct 14, 2000·Journal of Molecular Biology·J Huang, R Sousa
Apr 9, 2001·Nucleic Acids Research·X Wu, N Maizels
Jul 4, 2001·Nucleic Acids Research·P MohagheghI D Hickson
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·H SunN Maizels
Nov 10, 2001·Annual Review of Genomics and Human Genetics·C J Cummings, H Y Zoghbi
Jun 4, 2002·Nature Reviews. Molecular Cell Biology·James C Wang
Jun 7, 2002·Nature·Gary N ParkinsonStephen Neidle

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Citations

Dec 15, 2012·Journal of Computer-aided Molecular Design·Jin-Qiang HouZhi-Shu Huang
Mar 19, 2011·Molecular Biotechnology·Tracy M Bryan, Peter Baumann
Jun 7, 2005·Trends in Biochemical Sciences·Peter M Lansdorp
Jun 21, 2006·Biochemistry·Himesh FernandoShankar Balasubramanian
Sep 27, 2007·ACS Chemical Biology·W David Wilson, Hiroshi Sugiyama
Apr 20, 2013·Chemical Reviews·Hélène GaillardAndrés Aguilera
Aug 27, 2013·Chemical Reviews·Boris P BelotserkovskiiPhilip C Hanawalt
Feb 15, 2012·Nature Reviews. Genetics·Nayun Kim, Sue Jinks-Robertson
Oct 4, 2012·Nature Reviews. Genetics·Matthew L BochmanVirginia A Zakian
Nov 22, 2008·Nature Reviews. Molecular Cell Biology·Philip C Hanawalt, Graciela Spivak
Dec 6, 2006·Nature Structural & Molecular Biology·Nancy Maizels
Jun 24, 2008·Chemical Society Reviews·Julian Leon Huppert
Oct 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Aarthy C Vallur, Nancy Maizels
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Boris P BelotserkovskiiPhilip C Hanawalt
Feb 23, 2008·The Journal of Biological Chemistry·Silvia TornalettiPhilip C Hanawalt
May 13, 2009·The Journal of Experimental Medicine·Deepa RajagopalPatricia J Gearhart
Jul 30, 2005·Nucleic Acids Research·Christine GranotierFrançois D Boussin
Aug 18, 2006·Nucleic Acids Research·Johanna Eddy, Nancy Maizels
Sep 16, 2006·Nucleic Acids Research·Tonatiuh Romero SalasCarole Saintomé
Oct 3, 2006·Nucleic Acids Research·Sarah BurgeStephen Neidle
Mar 31, 2007·Nucleic Acids Research·Anne De Cian, Jean-Louis Mergny
Dec 26, 2007·Nucleic Acids Research·Jun TangZheng Tan
Oct 6, 2007·Nucleic Acids Research·Ren ZhangChun-Ting Zhang
Nov 30, 2007·Nucleic Acids Research·Oleg KikinParamjeet S Bagga
Aug 23, 2008·Nucleic Acids Research·Andrew N LaneJohn O Trent
Mar 27, 2009·Nucleic Acids Research·Júlia ViladomsCarlos González
Aug 22, 2009·Nucleic Acids Research·Kelly J NeavesJ Michael Edwardson
Sep 18, 2009·Nucleic Acids Research·Zhuo DuNing Li
Oct 13, 2009·Nucleic Acids Research·Mykola I OnyshchenkoIgor G Panyutin

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