Abstract
Experiments were carried out to test the hypothesis whether adenosine produced by ATP catabolism during ischemia can be trapped with L-homocysteine and be re-utilized during reperfusion. During intraatrial infusion of L-homocysteine (100 mg/kg/h), the ischemic accumulation of adenine nucleosides and oxypurines in dog myocardium was found to be less than 50% of that during control ischemia. A high proportion of adenosine was recovered as S-adenosyl-L-homocysteine. On reperfusion, S-adenosyl-L-homocysteine. On reperfusion, S-adenosyl-L-homocysteine tissue content remained high. After 3 hours of reperfusion approximately 50% of the accumulated S-adenosyl-L-homocysteine were still found in the tissue. Infusion of L-homocysteine did not cause an accumulation of S-adenosyl-L-homocysteine in the nonischemic myocardial tissue. L-homocysteine treatment caused a further depletion of ATP during reperfusion after 30 minutes of ischemia, which can be interpreted as a toxic effect. We conclude that L-homocysteine is indeed able to trap adenosine produced by ATP breakdown, but the reaction is not readily reversible and is therefore not useful for quick restoration of postischemic ATP levels.
References
Oct 18, 1979·Biochimica Et Biophysica Acta·P M Ueland, J Saebø
Jun 1, 1979·Journal of Molecular and Cellular Cardiology·J SchaperW Schaper
Sep 18, 1979·Biochemistry·P M Ueland, J Saebø
Mar 1, 1978·Circulation Research·R A OlssonM K Gentry
Nov 17, 1978·Science·M S Hershfield, N M Krodich
Mar 1, 1975·Canadian Journal of Biochemistry·R D Walker, J A Duerre
Feb 1, 1985·Circulation Research·M MauserW Schaper
May 1, 1973·Circulation Research·H G ZimmerE Gerlach
Sep 1, 1968·The American Journal of Cardiology·A Wollenberger, E G Krause
Jan 1, 1971·Cardiology·S Gudbjarnason
Sep 1, 1968·Circulation Research·W BraaschR J Bing
Nov 9, 1982·Biochimica Et Biophysica Acta·S O Døskeland, P M Ueland
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R A KlonerE Braunwald
Jun 1, 1982·Analytical Biochemistry·S H Payne, B N Ames
Jul 1, 1982·Circulation Research·J L SwainE W Holmes
Oct 1, 1981·Circulation Research·R B JenningsS E Mayer
Apr 15, 1981·The Biochemical Journal·J SchraderH Bardenheuer
Nov 1, 1964·Circulation Research·S IMAIR M BERNE
Citations
Sep 5, 2002·Circulation·Ya-Fei ChenAi-Ping Zou
Jul 1, 1988·Circulation Research·A DeussenJ Schrader
Mar 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·V M Sciotti, D G Van Wylen
Oct 4, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Niels P RiksenPaul Smits
Jan 1, 1990·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·Z GrunbaumK A Krohn