PMID: 6969289Oct 1, 1980Paper

Intracellular voltage of isolated epithelia of frog skin: apical and basolateral cell punctures

The Journal of General Physiology
R S FisherS I Helman

Abstract

Isolated epithelia of frog skin were prepared with collagenase, and the cells were punctured with intracellular microelectrodes across their apical (outer) and basolateral (inner) surfaces. Regardless of the route of cell puncture, the intracellular voltage (Vosc) in short-circuited isolated epithelia was markedly negative, averaging -70.4 mV for apical punctures and -91.6 mV for basolateral punctures. As in intact epithelia, amiloride outside caused the Vosc to become more negative (means of -96.7 and -101.8 mV), with a concomitant increase in the resistance of the apical barrier. Increasing the [K)i of the basolateral solution from 2.4 to 8.0 or 14.4 mM caused rapid step depolarization (5-10 s) of the Vosc under transepithelial Na transporting and amiloride-inhibited conditions of Na transport, with the delta Vosc ranging between 23.9 and 68.3 mV per decade change of [K]i. The finding that the Vosc of isolated epithelia of frog skin is independent of the route of cell penetration is consistent with the notion that the cells of the stratified epithelium are electrically coupled (functional syncitium). Moreover, the isolated epithelium can serve as a useful preparation, especially in studies designed to investigate the properti...Continue Reading

Citations

Dec 1, 1986·Pflügers Archiv : European journal of physiology·A I Kaufman, D Erlij
Jan 1, 1985·The Journal of Membrane Biology·E KelepourisM M Civan
May 1, 1988·The Journal of Membrane Biology·S LeibowichM M Civan
Apr 1, 1991·The Journal of Membrane Biology·M M CivanT G O'Brien
Jan 1, 1984·The Journal of Membrane Biology·J DeLong, M M Civan
Feb 1, 1991·The Journal of General Physiology·R S Fisher, W Van Driessche
Jul 1, 1991·The Journal of General Physiology·J F García-Díaz
Jan 1, 1983·Critical Reviews in Clinical Laboratory Sciences·A D Macknight, J P Leader
Mar 1, 1997·The American Journal of Physiology·B L Blazer-Yost, S I Helman
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Oct 1, 1989·The American Journal of Physiology·S H Shahin, J T Blankemeyer
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Mar 1, 1989·The American Journal of Physiology·M M CivanT G O'Brien
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Jul 1, 1982·The American Journal of Physiology·L E LinM M Civan

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