Intracerebral hemorrhage induces monocyte-related gene expression within six hours: Global transcriptional profiling in swine ICH

Metabolic Brain Disease
Kyle B WalshOpeolu Adeoye

Abstract

Intracerebral hemorrhage (ICH) is a severe neurological disorder with no proven treatment. Our prior research identified a significant association with monocyte level and ICH mortality. To advance our understanding, we sought to identify gene expression after ICH using a swine model to test the hypothesis that ICH would induce peripheral blood mononuclear cell (PBMC) gene expression. In 10 pigs with ICH, two PBMC samples were drawn from each with the first immediately prior to ICH induction and the second six hours later. RNA-seq was performed with subsequent bioinformatics analysis using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Ingenuity® Pathway Analysis (IPA). There were 182 significantly upregulated and 153 significantly down-regulated differentially expressed genes (DEGs) after ICH. Consistent with findings in humans, significant GO and KEGG pathways were primarily related to inflammation and the immune response. Five genes, all upregulated post-ICH and known to be associated with monocyte activation, were repeatedly DEGs in the significant KEGG pathways: CD14, TLR4, CXCL8, IL-18, and CXCL2. In IPA, the majority of upregulated disease/function categories were related to inflammation and immun...Continue Reading

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Citations

Sep 12, 2020·International Journal of Molecular Sciences·Marc Melià-SorollaTeresa Gasull
Mar 5, 2021·Frontiers in Immunology·Shuhao MeiJianmin Zhang

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Datasets Mentioned

BETA
GSE124624

Methods Mentioned

BETA
RNA-seq
blood collection

Software Mentioned

DESeq2
KOBAS
IPA
Ingenuity Pathway Analysis ( IPA )
Ingenuity ® Pathway Analysis ( IPA
R
DESeq2 package
Bioconductor

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