Intracerebroventricular injection of corticotropin-releasing hormone receptor antagonist blocks the suppression of pulsatile luteinizing hormone secretion induced by neuromedin U in ovariectomized rats after 48 hours of fasting

Neuroscience Letters
Hong QuanFukuko Kimura

Abstract

We recently reported that neuromedin U (NMU) and fasting synergistically suppressed the pulsatile LH secretion, even though NMU has been shown to act as a satiety factor. In the present study, we examined whether this synergistic effect on the pulsatile LH secretion was mediated via corticotropin-releasing hormone (CRH) neurons. Adult ovariectomized (OVX) rats were stereotaxically implanted with a guide cannula into the third ventricle. After 2 weeks of recovery, blood samples were taken under freely-moving conditions at 6-min intervals for 180 min from 09:00 to 12:00 h in OVX rats that had been fasted for 48 h. After first 60 min of blood sampling, astressin (2 nmol/3 microl), a CRH receptor antagonist, dissolved in artificial cerebrospinal fluid (aCSF) or aCSF (3 microl) was injected as a control into the third ventricle. Thirty minutes after the first injection, the rats were injected with NMU (1 nmol/3 microl) into the third ventricle. We found that pre-treatment with astressin completely blocked the prolongation of the interpulse interval, which should be induced by NMU. We confirmed that a single intracerebroventricular injection of astressin per se did not affect the pulsatile LH secretion. The present study suggests tha...Continue Reading

References

Aug 1, 1987·The Journal of Clinical Endocrinology and Metabolism·D H Olster, M Ferin
Sep 1, 1996·The Journal of Clinical Investigation·M W SchwartzD G Baskin
Oct 12, 2000·Biochemical and Biophysical Research Communications·M NakazatoS Matsukura
Nov 16, 2001·Biochemical and Biophysical Research Communications·R HanadaT Sakata
Dec 12, 2001·The Journal of Endocrinology·B J HolmbergD H Keisler
Apr 6, 2002·Preparative Biochemistry & Biotechnology·T Abiko, Y Takamura
Sep 20, 2002·Endocrinology·Tina R IvanovSimon M Luckman
Oct 26, 2002·Endocrinology·A M WrenS R Bloom
Feb 13, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Hilary DobsonRobert Smith
Mar 22, 2003·The Journal of Pharmacology and Experimental Therapeutics·Koki InoueEric P Zorrilla
Nov 19, 2003·Biochemical and Biophysical Research Communications·Hong QuanFukuko Kimura

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Citations

Nov 30, 2004·Neuroscience Letters·Manoel Baldoino Leal FilhoKonradin Metze
Jul 15, 2015·Journal of Molecular Endocrinology·Shuisheng LiHaoran Lin
Aug 4, 2009·Fundamental & Clinical Pharmacology·S Budhiraja, A Chugh
Jun 13, 2009·British Journal of Pharmacology·J D MitchellA P Davenport
Feb 15, 2019·International Journal of Toxicology·Melvin R WhiteThomas P Sanderson
Jan 22, 2015·Clinical Chemistry·Vanesa G Martinez, Lorraine O'Driscoll
Nov 18, 2006·Endocrinology·E VigoM Tena-Sempere
Aug 30, 2007·American Journal of Physiology. Endocrinology and Metabolism·Eva VigoManuel Tena-Sempere

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