Intracoronary Levosimendan during Ischemia Prevents Myocardial Apoptosis.

Frontiers in Physiology
M MalmbergTimo Savunen

Abstract

Levosimendan is a calcium sensitizer that has been shown to prevent myocardial contractile depression in patients post cardiac surgery. This drug exhibits an anti-apoptotic property; however, the underlying mechanism remains elusive. In this report, we characterized the myocardial protective of levosimendan in preventing cardiomyocyte apoptosis and post-operative stunning in an experimental ischemia-reperfusion model. Three groups of pigs (n = 8 per group) were subjected to 40 min of global, cardioplegic ischemia followed by 240 min of reperfusion. Levosimendan (65 μg/kg body weight) was given to pigs by intravenous infusion (L-IV) before ischemia or intracoronary administration during ischemia (L-IC). The Control group did not receive any levosimendan. Echocardiography was used to monitor cardiac function in all groups. Apoptosis levels were assessed from the left ventricle using the terminal transferase mediated dUTP nick end labeling (TUNEL) assay and immunocytochemical detection of Caspase-3. Pigs after ischemia-reperfusion had a much higher TUNEL%, suggesting that our treatment protocol was effective. Levels of apoptosis were significantly increased in Control pigs that did not receive any levosimendan (0.062 ± 0.044%) rel...Continue Reading

Citations

Aug 21, 2013·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Beyzagul PolatSabri Selcuk Atamanalp
Nov 18, 2017·PloS One·Stefanie N BrunnerAndres Beiras-Fernandez
May 3, 2014·Journal of Cardiovascular Pharmacology·Hai-yan ZhouYue-Liang Shen
Oct 24, 2019·BioMed Research International·Jian PanYuan-Qiang Lu

❮ Previous
Next ❯

Methods Mentioned

BETA
coronary
bypass
biopsies

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Related Papers

European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
M MalmbergT Savunen
Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Jialong GuoShunqing Zuo
© 2022 Meta ULC. All rights reserved