PMID: 9160859Apr 1, 1997Paper

Intracrine and autocrine effects of basic fibroblast growth factor in vascular smooth muscle cells

Journal of Molecular and Cellular Cardiology
M G DavisG W Dorn

Abstract

In order to elucidate the effects of the different basic fibroblast growth factor (bFGF) isoforms on vascular smooth muscle, we examined aorta-derived vascular smooth muscle cells from transgenic mice expressing the human isoforms of bFGF. Four cell lines were examined from mice in which transgene expression was driven by the ubiquitous phosphoglycerate kinase promoter. Overexpression and cellular localization was confirmed by Western blot analysis in vascular smooth muscle cells from mice expressing: all four human bFGF isoforms (24, 22, 21, and 18 kDa); all three nuclear targeted isoforms (24, 22, and 21 kDa); only the 24 kDa isoform; and the only secreted/non-nuclear targeted isoform, 18 kDa. All lines showed approximate four-fold increases in bFGF expression, nuclear localization of all nuclear targeted bFGF isoforms, and cytosolic localization of only the 18 kDa bFGF. Measurement of [3H]thymidine incorporation into quiescent cells stimulated with increasing concentrations of serum, showed increased DNA synthesis in cell lines expressing any bFGF isoform when compared to non-transgenic control cells, and a further increase in DNA synthesis in cells expressing the nuclear targeted isoforms (24, 22, and 21 kDa) over the 18 kD...Continue Reading

Citations

Sep 2, 2003·Cytokine & Growth Factor Reviews·Mohamad AzharThomas Doetschman
Feb 14, 1998·Nature Medicine·M ZhouT Doetschman
Jun 2, 1998·Kidney International·P Carmeliet, D Collen
Sep 3, 2002·American Journal of Respiratory and Critical Care Medicine·Yoshikazu InoueLee S Newman
Apr 20, 2000·The Journal of Clinical Investigation·A MonteroM M Hurley
Nov 22, 2001·Biochemical and Biophysical Research Communications·F SouletG Bouche
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·H NaoraR B Roden
May 4, 1999·The Journal of Cell Biology·M Keresztes, J Boonstra
Sep 6, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Siyun LiaoJo El J Schultz
Dec 24, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mohamad AzharTom Doetschman
Feb 2, 2010·Journal of Molecular and Cellular Cardiology·Siyun LiaoJo El J Schultz
Feb 3, 2018·Molecular Reproduction and Development·Yasmin SchuermannRaj Duggavathi
Apr 4, 2000·Cell Death and Differentiation·C GiampietriC Gaetano
Nov 1, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kerstin AmannHeimo Ehmke
Jun 27, 2018·Journal of the Endocrine Society·J Douglas CoffinMarja Marie Hurley
Apr 27, 2019·Cartilage·Parvathy ThampiJames N MacLeod
Jan 6, 2021·International Journal of Molecular Sciences·Friederike Freiin von HövelClaudia Grothe

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