Intracytoplasmic sperm injection affects embryo developmental potential and gene expression in cattle

Reproductive Biology
María Elena AriasRicardo Felmer

Abstract

Some reports have linked intracytoplasmic sperm injection (ICSI) with chromosomal abnormalities, low developmental potential and altered gene expression in embryos. ICSI has also been linked with obesity, early aging and increased incidence of tumors in offspring. Other reports have demonstrated that some of these complications disappeared within a few weeks of life or even showed a lack of such associations. The aim of this study was to evaluate and compare embryo development, quality and gene expression in bovine embryos generated by ICSI and by conventional in vitro fertilization (IVF) insemination. The results showed differences in cleavage (88.5% in IVF and 64.1% in ICSI) and blastocyst formation rates (36.1% in IVF and 22.3% in ICSI). The proportion of ICM cells to total cell count was higher in ICSI (39.2%) than in IVF embryos (29.5%). However, no differences were observed in the total embryonic cell numbers (159.3±28.5 and 161.2±56.2 for IVF and ICSI, respectively) or in the proportion of apoptotic nuclei to the total embryonic cell numbers (2.12 and 2.64% for IVF and ICSI, respectively). Gene expression analysis showed a down-regulation of insulin-like growth factor 2 (IGF2) and overexpression of bcl-2-like protein 4 (...Continue Reading

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Citations

May 4, 2016·Zygote : the Biology of Gametes and Early Embryos·María Elena AriasRicardo Felmer
Nov 1, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·A P López-CardonaA Gutierrez-Adán
May 18, 2017·Molecular Reproduction and Development·Luis ÁguilaRicardo Felmer
Dec 3, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Daniel F SalamoneMaría Belén Rodríguez
Aug 11, 2017·Clinical and Experimental Reproductive Medicine·Caizhu WangRuoyun Lin
Jul 10, 2020·Scientific Reports·Minjie TanBernard A J Roelen
Feb 8, 2021·Biology of Reproduction·Cecilia ValenciaMaría Elena Arias
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Luis AguilaLawrence C Smith

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