Intracytoplasmic sperm injection of frozen-thawed bovine oocytes and subsequent embryo development

Molecular Reproduction and Development
Gyu-Jin RhoSang-Young Choe

Abstract

Oocyte cryopreservation and intracytoplasmic sperm injection (ICSI) are advantageous to expand their usefulness in genetic engineering. Oocytes matured for 22 hr were vitrified in droplets of cryoprotectants (3.2 M ethylene glycol (EG), 2.36 M dimethyl sulfoxide (DMSO), 0.6 M sucrose) on copper electron microscope (EM) grids. After being warmed, the oocytes were cultured in IVM medium for an additional 2 hr. Sperm treated with dithiothreitol were utilized for ICSI. Oocytes injected with sperm were activated by combination of ionomycin with cycloheximide (CHX). The ICSI oocytes were compared for the rates of pronuclear formation, development, cell number, and the ratio of ICM to those of fresh ICSI and IVF control. The proportion of 2PN formation was significantly higher in IVF control (Group 1) than those in other treated groups. Among the treated groups a significant lower 2PN formation was observed in IVF-frozen-thawed than in ICSI-fresh and frozen-thawed groups. Cleavage rates in IVF-frozen-thawed and ICSI-frozen-thawed groups were significantly lower than those of IVF control and ICSI-fresh groups. In ICSI groups, the rates of cleavage and blastocyst in fresh oocytes were significantly higher than in frozen-thawed. Developm...Continue Reading

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Nov 2, 2002·Molecular Reproduction and Development·Gyu-Jin RhoSang-Yong Choe

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Citations

Aug 12, 2014·Science China. Life Sciences·Mahmoud MoussaFanYi Zeng
Oct 29, 2008·Reproduction in Domestic Animals = Zuchthygiene·E García-RosellóM A Silvestre
Jun 15, 2012·Reproduction in Domestic Animals = Zuchthygiene·C DíezE Gómez
Aug 10, 2006·Animal Reproduction Science·Lian-Yu ShiGyu-Jin Rho
Oct 20, 2005·Theriogenology·Keith J Betteridge
Mar 27, 2012·Theriogenology·S M HosseiniM H Nasr-Esfahani
May 18, 2017·Molecular Reproduction and Development·Luis ÁguilaRicardo Felmer
Jul 29, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Luis ÁguilaRafael Fissore
Oct 6, 2017·Biopreservation and Biobanking·Guobo QuanQionghua Hong
Sep 12, 2007·Human Reproduction Update·Debra A Gook, David H Edgar
Feb 13, 2021·Genes·Veena UnnikrishnanJacob Thundathil

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