PMID: 107957Feb 1, 1979

Intracytoplasmic type A particles from mammary tumours and leukaemias of strain ICRC mice

British Journal of Cancer
K A KarandeK J Ranadive

Abstract

The ovarian-hormone-induced leukaemias of strain ICRC mice, with an abundance of intracytoplasmic type A particles in primary as well as transplanted lesions, were used to study morphological, biophysical, immunological and structural characteristics of type A particles. Mammary tumours of strains ICRC and C3H(Jax) were also used as sources for type A particles. The purified virions banded at the density of 1.20 g/ml in 12--60% linear sucrose-density gradient when subjected to spinning at 113,000 g for 4 h. The SDS-polyacrylamide-gel electrophoresis of type A particles from mammary tumours and leukaemias reproducibly resolved at least 8 polypeptides, 2 of these 54,000 and 24,000 dalton proteins, showing variable expression. Type A particles and B particles, despite the fact that each had a distinct polypeptide pattern, showed common antigens with different electrophoretic mobilities. Proteins of 24,000, 18,000 and 12,000 daltons from B particles were found to be antigenically related to those from type A particles. The bioassay studies carried out with purified A particles showed that 2/7 males of strain ICRC and 1/6 females of strain DBA-MTI developed leukaemias, as against none in the controls, when inoculated between the age...Continue Reading

Related Concepts

Centrifugation, Density Gradient
Protoplasm
Immunodiffusion Measurement
Immunoelectrophoresis
Negri Body
Leukemia, Experimental
Mammary Neoplasms, Experimental
Mice, Inbred Strains
Electron Microscopy
Neoplasm Transplantation

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