Intradermal DNA vaccination in ear pinnae is an efficient route to protect cats against rabies virus

Veterinary Research
Emiliano Tesoro-CruzA Aguilar-Setien

Abstract

A DNA vaccine against rabies (pGQH) was administrated to cats in order to examine different administration routes. Four groups of three cats each were inoculated with pGQH as follows: group A, intramuscularly (IM), 100 microg; group B, intranasally (IN), 100 microg; group C, intradermally into ear pinnae (ID-EP), 100 microg, and group D, IM, 200 microL of phosphate buffer solution (PBS) alone (control group). Blood was drawn on days 0, 30, 60, 90, 120, 150, and 180. Groups A, B, and C received a booster on day 30. At day 200 all animals were challenged. A passive transfer of cat sera, as well as a viral challenge, was performed in mice. The results displayed that neutralizing antibody titers were higher in cats of group C (ID-EP) showing high early titers (> 2 IU) and the highest titer was on day 120 (> 14 IU). In group B (IN), two out of three cats seroconverted on day 30 (> 0.5 IU), the third cat seroconverted until day 60 (> 0.5 IU). In contrast, the lowest levels of neutralizing antibodies were detected in group A (IM). The control group showed no anti-rabies antibodies. Groups A (IM) and D (control) succumbed after lethal challenge. All animals from the ID-EP group (C) survived, only one individual from the IN (B) group di...Continue Reading

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Citations

Apr 9, 2010·Viral Immunology·Elizabeth W UhlRobert J Hogan
Oct 13, 2015·Therapeutic Delivery·David ChenThean Yeoh
Oct 23, 2008·Vaccine·Emiliano Tesoro Cruz José Alvaro Aguilar Setién
Nov 19, 2018·Molecular Biotechnology·Alexandre SamoylovTatiana Samoylova
Sep 3, 2009·Expert Review of Vaccines·Laurel Redding, David B Weiner

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