Intrafibrillar, bone-mimetic collagen mineralization regulates breast cancer cell adhesion and migration

Biomaterials
Siyoung ChoiClaudia Fischbach

Abstract

Bone metastasis is a leading cause of death in patients with breast cancer, but the underlying mechanisms are poorly understood. While much work focuses on the molecular and cellular events that drive breast cancer bone metastasis, it is mostly unclear what role bone extracellular matrix (ECM) properties play in this process. Bone ECM primarily consists of mineralized collagen fibrils, which are composed of non-stoichiometric carbonated apatite (HA) and collagen type I. Reduced bone mineral content is epidemiologically linked with increased risk of bone metastasis. Yet elucidating the potential functional impact of collagen mineralization on breast cancer cells has remained challenging because of a lack of model systems that allow studying tumor cell behavior as a function of physiological, intrafibrillar collagen mineralization. Here, we have developed cell culture substrates composed of mineralized collagen type I fibrils using a polymer-induced liquid-precursor (PILP) process. Intrafibrillar HA decreased breast cancer cell adhesion forces and accordingly reduced collagen fiber alignment relative to cells cultured on control collagen. The resulting mineral-mediated changes in collagen network characteristics and mechanosignal...Continue Reading

Citations

Feb 29, 2020·Tissue Engineering. Part B, Reviews·Ishani D PremaratneJason A Spector
Aug 5, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nicholas G FischerConrado Aparicio
Aug 23, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Noam Zuela-Sopilniak, Jan Lammerding
Nov 9, 2018·Biophysics Reviews·Vassilis PapalazarouLaura M Machesky
Dec 5, 2020·Advanced Healthcare Materials·Netta VidavskyLara A Estroff
Jan 2, 2021·Journal of Biomechanics·Victoria L ThaiJ Kent Leach
Sep 13, 2019·IScience·Apratim MukherjeeAmrinder S Nain
May 1, 2021·Cells·Gaël RunelIngrid Masse
Jul 2, 2021·Advanced Drug Delivery Reviews·Matthew L TanClaudia Fischbach
Jun 19, 2019·ACS Biomaterials Science & Engineering·Alexander J BoysLawrence J Bonassar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.