Intraflagellar Transport Complex B Proteins Regulate the Hippo Effector Yap1 during Cardiogenesis.

Cell Reports
Marina PeraltaJulien Vermot

Abstract

Cilia and the intraflagellar transport (IFT) proteins involved in ciliogenesis are associated with congenital heart diseases (CHDs). However, the molecular links between cilia, IFT proteins, and cardiogenesis are yet to be established. Using a combination of biochemistry, genetics, and live-imaging methods, we show that IFT complex B proteins (Ift88, Ift54, and Ift20) modulate the Hippo pathway effector YAP1 in zebrafish and mouse. We demonstrate that this interaction is key to restrict the formation of the proepicardium and the myocardium. In cellulo experiments suggest that IFT88 and IFT20 interact with YAP1 in the cytoplasm and functionally modulate its activity, identifying a molecular link between cilia-related proteins and the Hippo pathway. Taken together, our results highlight a noncanonical role for IFT complex B proteins during cardiogenesis and shed light on a mechanism of action for ciliary proteins in YAP1 regulation.

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T Mikawa, D A Fischman
Sep 11, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L RobbR P Harvey
Apr 9, 2001·Cell and Tissue Research·R CarmonaR Muñoz-Chápuli
Nov 5, 2002·Nature Reviews. Molecular Cell Biology·Joel L Rosenbaum, George B Witman
Jun 25, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jörg MännerThomas Brand
Nov 24, 2005·Nature Reviews. Genetics·Margaret BuckinghamStéphane Zaffran
May 9, 2006·Developmental Biology·Jan SchlueterThomas Brand
Dec 15, 2006·Development·Courtney J HaycraftBradley K Yoder
Jan 15, 2008·Nature Protocols·Christine Thisse, Bernard Thisse
Jun 27, 2008·Genes & Development·Bin ZhaoKun-Liang Guan
Aug 30, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jennifer SloughMartina Brueckner
Nov 5, 2008·The Journal of Cell Biology·Julie A JonassenGregory J Pazour
Jul 16, 2009·Current Biology : CB·Nicolas F BerbariBradley K Yoder
Aug 25, 2009·Development·Peng Huang, Alexander F Schier
Nov 26, 2009·Virology·Zifei PeiAnthony P Schmitt
Dec 17, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Shang Yew TaySudipto Roy
Jan 30, 2010·Cardiovascular Research·Mathilda T M MommersteegVincent M Christoffels
Mar 23, 2010·Nature Cell Biology·Antonia BorovinaBrian Ciruna
Apr 24, 2010·Circulation Research·Jiandong Liu, Didier Y R Stainier
Jun 26, 2010·Journal of the American Society of Nephrology : JASN·Ying CaoZhaoxia Sun
Oct 13, 2010·Pharmacology & Therapeutics·Federica LimanaAntonia Germani
Dec 29, 2010·The Journal of Biological Chemistry·Wenqi WangJunjie Chen
Jan 6, 2011·Genes & Development·Bin ZhaoKun-Liang Guan
Mar 29, 2011·Nature Cell Biology·Benedicte DelavalStephen Doxsey
May 10, 2011·Nature Genetics·Polloneal Jymmiel R OcbinaKathryn V Anderson
May 24, 2011·Nature Cell Biology·Madeline A LancasterJoseph G Gleeson
Nov 29, 2011·Differentiation; Research in Biological Diversity·Michael TaschnerEsben Lorentzen
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona

❮ Previous
Next ❯

Citations

Oct 20, 2020·Frontiers in Cell and Developmental Biology·Benjamin VitreBenedicte Delaval
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Yong Suk Cho, Jin Jiang
May 1, 2021·International Journal of Molecular Sciences·Fang GengRuilin Zhang
Aug 24, 2021·Frontiers in Cell and Developmental Biology·Dorothee Bornhorst, Salim Abdelilah-Seyfried

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.