Sep 30, 2016

Intraflagellar transport protein IFT20 is essential for male fertility and spermiogenesis in mice

Molecular Biology of the Cell
Zhengang ZhangZhibing Zhang

Abstract

Intraflagellar transport (IFT) is a conserved mechanism thought to be essential for the assembly and maintenance of cilia and flagella. However, little is known about its role in mammalian sperm flagella formation. To fill this gap, we disrupted the Ift20 gene in male germ cells. Homozygous mutant mice were infertile with significantly reduced sperm counts and motility. In addition, abnormally shaped elongating spermatid heads and bulbous round spermatids were found in the lumen of the seminiferous tubules. Electron microscopy revealed increased cytoplasmic vesicles, fiber-like structures, abnormal accumulation of mitochondria and a decrease in mature lysosomes. The few developed sperm had disrupted axonemes and some retained cytoplasmic lobe components on the flagella. ODF2 and SPAG16L, two sperm flagella proteins failed to be incorporated into sperm tails of the mutant mice, and in the germ cells, both were assembled into complexes with lighter density in the absence of IFT20. Disrupting IFT20 did not significantly change expression levels of IFT88, a component of IFT-B complex, and IFT140, a component of IFT-A complex. Even though the expression level of an autophagy core protein that associates with IFT20, ATG16, was reduce...Continue Reading

Mentioned in this Paper

Biological Markers
Fertility
Study
Benign Neoplasm of Testis
Isoxaflutole
IFT88 gene
Entire Lumen of Body System
Cell Motility
Cytoplasmic Vesicles
Axonemal Dyneins

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