Intragenic homozygous deletions of MTS1 gene in gastric cancer in Taiwan

Japanese Journal of Cancer Research : Gann
M S WuJ T Lin

Abstract

The multiple tumor suppressor 1 (MTS1) and 2 (MTS2) genes, located on chromosome 9p21, have been reported to be deleted or mutated in many malignant cell lines and in a high percentage of some primary carcinomas. To determine whether these genes are altered, and if so, what is the nature of the alterations, in human gastric adenocarcinoma, we investigated their frequency of mutation by Southern blotting, polymerase chain reaction (PCR) and direct sequencing in 55 patients. Furthermore, loss of heterozygosity (LOH) of chromosome 9p21 at the IFNA locus and D9S171 was assessed. Homozygous deletions of exon 1 of the MTS1 gene were identified in 5 of 55 (9.1%) primary tumors. No deletion of MTS2 gene was noted. LOH was observed in 7 (14.3%) of 49 informative cases (5 cases at IFNA locus, 2 cases at D9S171 and one case with combined LOH at D9S171 and homozygous deletion at exon 1 of MTS1). Direct sequencing of PCR products of the MTS1 and MTS2 gene did not reveal any point mutation in these 55 patients. These data indicate that alterations of the MTS1 and MTS2 genes are infrequently encountered. Additional studies of LOH with more microsatellite markers near 9p21 are mandatory to elucidate whether another tumor suppressor gene exists...Continue Reading

References

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Citations

Mar 19, 2013·Journal of the Chinese Medical Association : JCMA·Peng WangBaoguo Liu
Sep 30, 2008·International Journal of Cancer. Journal International Du Cancer·Cai-Xuan DongWei-Cheng You
Mar 10, 2001·Experimental Cell Research·J W Rocco, D Sidransky
Jul 25, 2015·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Ioana CutcutacheSteven G Rozen
Mar 15, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Daya LuoDajun Deng
Jun 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·Y H ShimJ Y Ro
Aug 7, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yu SunJi-You Li
Nov 21, 1998·Biochimica Et Biophysica Acta·M Ruas, G Peters

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