Intragenomic variation of fungal ribosomal genes is higher than previously thought

Molecular Biology and Evolution
Uwe K Simon, Michael Weiss

Abstract

Nuclear ribosomal genes in most eukaryotes are present in multiple copies and often used for taxonomic and phylogenetic analyses. We comprehensively examined intragenomic polymorphism levels of three nuclear ribosomal loci for four important plant pathogenic fungi by polymerase chain reaction amplification and cloning. Here, we show that single nucleotide polymorphisms are present in an unexpectedly high amount. This might have implications for studies of fungal evolution, phylogenetics, and population genetics. Furthermore, our work demonstrates that the majority of all ribosomal sequences obtained from one individual and gene is identical to the majority rule consensus sequence of all detected sequence variants. Due to the large number of polymorphisms found and the fact that the polymorphism level differed markedly even between ribosomal genes of one and the same individual, we assume that nuclear ribosomal genes might not always evolve in a strictly concerted manner.

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Citations

Apr 21, 2010·Applied Microbiology and Biotechnology·Dominik BegerowWolfgang Maier
May 9, 2013·Molecular Biotechnology·Mukhamad Su'udiIl-Pyung Ahn
Mar 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Conrad L SchochUNKNOWN Fungal Barcoding Consortium Author List
Jun 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Levente Kiss
Feb 10, 2012·Molecular Biology and Evolution·Raquel Rodríguez-MartínezRamon Massana
Apr 2, 2011·BMC Research Notes·Ayalew B MentewabRebekah A Flowers
Oct 23, 2013·PloS One·Holly M BikW Kelley Thomas
Jul 2, 2014·Database : the Journal of Biological Databases and Curation·Conrad L SchochScott Federhen
Jul 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Robert LückingJames D Lawrey
Oct 28, 2015·International Journal of Molecular Sciences·Ying ZhaoPatrick C Y Woo
Apr 10, 2012·Fungal Biology·Yanchun WangTom Hsiang
Dec 24, 2015·Fungal Biology·Hildrun WalterUwe K Simon
May 12, 2012·American Journal of Physical Anthropology·Peter ValloDavid Modrý
Feb 28, 2016·Antonie van Leeuwenhoek·Elke J van NieuwenhuijzenRobert A Samson
Mar 21, 2015·Microbial Ecology·Antonia FleischhackerLucia Muggia

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