Intraglomerular C3 synthesis in human kidney detected by in situ hybridization.

Journal of the American Society of Nephrology : JASN
M MiyazakiH Sakai

Abstract

Complements 3 and 4 are known to be synthesized in diseased renal tissue and the mRNA of these complements has been demonstrated, using polymerase chain reaction, in renal biopsies from nephritic patients. However, the types of cells producing the complements in intact renal tissue have not been defined. To identify the renal cellular components involved in complement synthesis, we analyzed the expression of C3 mRNA in renal tissues from patients with immune-complex glomerulonephritis by a high-resolution in situ hybridization using digoxigenin-labeled oligonucleotide. Renal tissues from 15 patients with immunoglobulin (Ig) A nephropathy (IgAN), five with lupus nephritis (LN), and five with minimal change nephrotic syndrome (MCNS) were examined. Uninvolved portions of surgically removed kidney with tumors served as normal controls. C3 mRNA was detected in mesangial cells, glomerular epithelial cells, and Bowman's capsule in IgAN and LN. In the interstitium, some tubules and some infiltrating mononuclear cells were positively stained for C3 mRNA. C3 mRNA was not detected in MCNS and control tissues. Our results confirm that the glomerular resident cells can synthesize C3 in immune-mediated glomerulonephritis and suggest that loc...Continue Reading

Citations

Jun 9, 1998·Current Opinion in Nephrology and Hypertension·N S Sheerin, S H Sacks
Mar 22, 2001·Kidney International·W ZhouS H Sacks
May 5, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Masato KimuraAkira Hishida
Jul 28, 2006·Molecular Immunology·Wuding ZhouSteven H Sacks
Feb 18, 2003·The Journal of Clinical Investigation·Sydney TangKar Neng Lai
Jul 13, 1999·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·A O MudaT Faraggiana
Sep 12, 2000·The American Journal of Pathology·J R PrattS H Sacks

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