Intraluminal antibodies to macrophage migration inhibitory factor decrease substance P induced inflammatory changes in the rat bladder and prostate

The Journal of Urology
K Meyer-Siegler, Pedro L Vera

Abstract

Noxious stimuli induce substance P (SP) secretion from nerve terminals, resulting in plasma extravasation, edema and hyperalgesia, commonly referred to as neurogenic inflammation. Since SP is a short-lived molecule, additional proinflammatory mediators maintain continued inflammation. The bladder contains stores of preformed macrophage migration inhibitory factor (MIF), a proinflammatory cytokine, which is released into the lumen in response to SP. MIF may act in an amplifying manner to maintain or increase inflammation. Inducing inflammatory changes with SP, while sequestering released luminal MIF with an antibody, tested this hypothesis. In anesthetized rats the ureters were cut to isolate the bladder and the bladder contents were replaced with saline or antiMIF antibody (5 or 15 microg/ml), immediately followed by systemic SP or saline. Changes in the expression of inflammatory cytokines, and histological changes in the bladder and prostate were evaluated 1 hour later. : Targeted array analysis identified increases in proinflammatory gene expression in the bladder and prostate as a result of SP. SP induced changes in MIF, cyclooxygenase-2, nerve growth factor, c-fos and edema were decreased by intraluminal anti-MIF. SP incre...Continue Reading

References

Mar 1, 1993·Canadian Journal of Physiology and Pharmacology·M NicolauD Regoli
Mar 4, 2000·European Journal of Pain : EJP·M A Giamberardino
Apr 6, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M A Vizzard
Nov 9, 2000·Clinical and Experimental Pharmacology & Physiology·S VenturaJ N Pennefather
Apr 5, 2002·Microbes and Infection·Hongqi LueJürgen Bernhagen
Jun 12, 2002·The American Journal of Pathology·Marcia R SabanRicardo Saban
Aug 17, 2002·The Journal of Pharmacology and Experimental Therapeutics·Jennelle Durnett Richardson, Michael R Vasko
Dec 24, 2003·Drug Discovery Today·Morag Robertson
Feb 26, 2004·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Katherine L Meyer-SieglerPedro L Vera
Mar 17, 2004·The Journal of Urology·Katherine L Meyer-Siegler, Pedro L Vera

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Citations

Oct 21, 2009·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Esra SevketogluSami Hatipoglu
Aug 20, 2009·Anesthesia and Analgesia·Asokumar BuvanendranMichael J Iadarola
Aug 21, 2010·The Journal of Urology·Kenneth A Iczkowski
Mar 18, 2009·Neurourology and Urodynamics·Simone D GueriosDale E Bjorling
Feb 4, 2010·Neurourology and Urodynamics·Pedro L VeraKatherine L Meyer-Siegler
Oct 26, 2011·The Prostate·Tiiu KullisaarReet Mändar
May 29, 2015·PloS One·Dimitrios E KouzoukasPedro L Vera
Jan 12, 2005·The Journal of Urology·Katherine L Meyer-Siegler, Pedro L Vera
Jul 1, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Haicheng ChenGuihua Liu

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