Intramammary 25-hydroxyvitamin D3 treatment modulates innate immune responses to endotoxin-induced mastitis

Journal of Dairy Science
Kathryn E MerrimanCorwin D Nelson

Abstract

Vitamin D signaling in response to pathogen-associated molecules contributes to activation of innate immune responses of bovine monocytes. We hypothesized that lipopolysaccharide (LPS) of bacteria associated with mastitis in dairy cows activates the vitamin D pathway in innate immune cells of the udder and that increasing availability of 25-hydroxyvitamin D3 [25(OH)D3] would augment expression of vitamin D-associated genes. The objective of this experiment was to determine the effects of intramammary LPS and 25(OH)D3 treatments on activation of the vitamin D pathway and innate immune responses of mammary immune cells. Individual mammary quarters of 5 lactating cows were treated with placebo control, 100 μg of 25(OH)D3, 5 μg of LPS, or a combination of 100 μg of 25(OH)D3 and 5 μg of LPS. Somatic cells from milk were evaluated for percentage of neutrophil and macrophage populations and expression of genes associated with vitamin D metabolism and innate immunity. Data from samples collected from 4 to 12 h after challenge were analyzed for main effects of LPS and 25(OH)D3 treatments, treatment interactions, and simple effects of 25(OH)D3 treatment. Data from samples collected at the time of challenge were used as covariates. The pe...Continue Reading

References

Dec 16, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Katinka StoffelsChantal Mathieu
Apr 14, 2006·Veterinary Research·Pascal Rainard, Céline Riollet
Jun 30, 2006·Veterinary Immunology and Immunopathology·John D LippolisRonald L Horst
Aug 12, 2008·The American Journal of Clinical Nutrition·Anthony W Norman
Oct 13, 2011·PloS One·John D LippolisCorwin D Nelson
Jun 6, 2012·The Veterinary Clinics of North America. Food Animal Practice·Pamela L Ruegg
Aug 10, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Kathryn E MerrimanCorwin D Nelson
Oct 17, 2016·Journal of Dairy Science·Corwin D NelsonWilliam P Weiss
Feb 24, 2017·The Journal of Steroid Biochemistry and Molecular Biology·Kathryn E MerrimanCorwin D Nelson

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Citations

Jan 30, 2021·The Veterinary Quarterly·Khan SharunWanpen Chaicumpa
Mar 15, 2021·Journal of Dairy Science·Rita Couto SerrenhoStephen J LeBlanc

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