Intramammary challenge of lipopolysaccharide stimulates secretion of lingual antimicrobial peptide into milk of dairy cows

Journal of Dairy Science
Naoki IsobeY Yoshimura

Abstract

Lingual antimicrobial peptide (LAP) belongs to the beta-defensin family in cattle and is found in bovine milk. However, it is unclear whether LAP is involved in the early immune response to mammary infection. The aim of the study was to investigate the changes of LAP concentration in milk after intramammary challenge with lipopolysaccharide (LPS), the gram-negative bacteria cell membrane component, in dairy cows. Milk was collected before and after LPS or phosphate-buffered saline (control) challenge every hour for 12 h on d 0 and twice daily from d 1 to 7. Somatic cell count (SCC), LAP concentration, and lactoperoxidase (LPO) activity in the milk were measured. Somatic cell count started to increase at 2 h postchallenge and remained high until d 5 (694 +/- 187 x 10(3 )to >1,000 +/- 0 x 10(3) cells/mL at d 0; >1,000 +/- 0 x 10(3) cells/mL at d 1 to 3; 684 +/- 194 x 10(3 )to 829 +/- 108 x 10(3 )cells/mL at d 4; 527 +/- 197 x 10(3 )to 656 +/- 145 x 10(3 )cells/mL at d 5). Somatic cell count increased in the control cows, although the levels were lower compared with those in the LPS challenge group. The LAP concentration in milk increased significantly at 2 h post-LPS-challenge and was maintained at high levels until d 2 (8.6 +/- ...Continue Reading

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Citations

Nov 23, 2016·Innate Immunity·Jeffery A CarrollPhillip A Lancaster
Apr 25, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Naoki Isobe
Mar 16, 2017·Reproduction in Domestic Animals = Zuchthygiene·K KuwaharaN Isobe
Jun 19, 2018·The Journal of Veterinary Medical Science·Yasunori ShinozukaNaoki Isobe
Jul 27, 2011·Journal of Dairy Science·N IsobeY Yoshimura
Sep 10, 2014·The Journal of Reproduction and Development·Naoki IsobeYukinori Yoshimura
Dec 4, 2015·Animal Science Journal = Nihon Chikusan Gakkaihō·Pongphol PongthaisongSittiruk Roytrakul
Apr 15, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Naoki IsobeYukinori Yoshimura
Apr 15, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Nana KitanoToshihiko Takahashi
Jul 28, 2015·Animal Science Journal = Nihon Chikusan Gakkaihō·Supreena SrisaikhamNaoki Isobe
Jul 12, 2017·The Journal of Veterinary Medical Science·Tomoko KoshiishiNaoki Isobe
May 14, 2018·Journal of Dairy Science·Kathryn E MerrimanCorwin D Nelson
Jun 15, 2017·Scientific Reports·Vincent HerryPascal Rainard
Jun 26, 2014·Animal Science Journal = Nihon Chikusan Gakkaihō·Kentaro NagaokaGen Watanabe
Jan 1, 2014·Journal of Veterinary Medicine·Mohamed Ezzat AlnakipJorge Barros-Velázquez
Nov 17, 2019·Veterinary Immunology and Immunopathology·Fika Yuliza PurbaNaoki Isobe
Apr 19, 2011·Veterinary Immunology and Immunopathology·Naoki IsobeYukinori Yoshimura
Nov 25, 2011·Veterinary Immunology and Immunopathology·Ya Qiong HuangNaoki Isobe
Nov 16, 2021·The Journal of Veterinary Medical Science·Naoki IsobeKeiichi Hisaeda
Jan 12, 2022·Journal of Mammary Gland Biology and Neoplasia·Yusaku TsugamiNaoki Isobe

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