Intramembrane charge movement and sarcoplasmic calcium release in enzymatically isolated mammalian skeletal muscle fibres

The Journal of Physiology
P SzentesiL Csernoch

Abstract

1. Single muscle fibres were dissociated enzymatically from the extensor digitorum longus and communis muscles of rats and guinea-pigs. The fibres were mounted into a double Vaseline gap experimental chamber and the events in excitation-contraction coupling were studied under voltage clamp conditions. 2. The voltage dependence of intramembrane charge movement followed a two-state Boltzmann distribution with maximal available charge of 26.1 +/- 1.5 and 26.1 +/- 1.3 nC microF-1, mid-point voltage of -35.1 +/- 5.0 and -42.2 +/- 1.2 mV and steepness of 16.7 +/- 2.2 and 17.0 +/- 1.9 mV (means +/- S.E.M., n = 7 and 4) in rats and guinea-pigs, respectively. 3. Intracellular calcium concentration ([Ca2+]i) was monitored using the calcium-sensitive dyes antipyrylazo III, fura-2 and mag-fura-5. Resting [Ca2+]i was similar in rats and guinea-pigs with 125 +/- 18 and 115 +/- 8 nM (n = 10 and 9), respectively, while the maximal increase for a 100 ms depolarization to 0 mV was larger in rats (6.3 +/- 1.0 microM; n = 7), than in guinea-pigs (2.8 +/- 0.3; n = 4). 4. The rate of calcium release (Rrel) from the sarcoplasmic reticulum (SR) displayed an early peak followed by a fast and a slow decline to a quasi maintained steady level. After norm...Continue Reading

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Citations

Feb 11, 2005·Naunyn-Schmiedeberg's Archives of Pharmacology·Henrietta SzappanosLászló Csernoch
Jun 15, 2007·Pflügers Archiv : European journal of physiology·Gyula Péter SzigetiIstván Jóna
May 7, 2008·Pflügers Archiv : European journal of physiology·Janos AlmassyIstvan Jona
Oct 5, 2001·The Journal of General Physiology·P SzentesiL Csernoch
Dec 18, 2013·The Journal of General Physiology·Zhiyong ZhuLeonid V Zingman
Jul 17, 2003·BMC Pharmacology·Norbert SzentandrássyLászló Csernoch
Mar 9, 2004·The Journal of Physiology·Christopher E WoodsJulio L Vergara
Nov 17, 2001·The Journal of Physiology·F FranciniR Squecco
Nov 4, 2000·The Journal of Physiology·P SzentesiL Csernoch
Jan 8, 2008·Biophysical Journal·Ramón Jiménez-MorenoOsvaldo Delbono
Nov 9, 2016·Nutrition & Metabolism·Dóra BodnárPéter Szentesi
Oct 22, 2008·Methods : a Companion to Methods in Enzymology·R Madelaine ParedesJames D Lechleiter
Oct 13, 2017·The Journal of General Physiology·Juan Ferreira GregorioGustavo Brum
Mar 1, 2014·Biophysics Reviews·Juan C CalderónCarlo Caputo

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