Intramembrane cleavage of microneme proteins at the surface of the apicomplexan parasite Toxoplasma gondii

The EMBO Journal
Corinna OpitzDominique Soldati

Abstract

Apicomplexan parasites actively secrete proteins at their apical pole as part of the host cell invasion process. The adhesive micronemal proteins are involved in the recognition of host cell receptors. Redistribution of these receptor-ligand complexes toward the posterior pole of the parasites is powered by the actomyosin system of the parasite and is presumed to drive parasite gliding motility and host cell penetration. The microneme protein protease termed MPP1 is responsible for the removal of the C-terminal domain of TgMIC2 and for shedding of the protein during invasion. In this study, we used site-specific mutagenesis to determine the amino acids essential for this cleavage to occur. Mapping of the cleavage site on TgMIC6 established that this processing occurs within the membrane-spanning domain, at a site that is conserved throughout all apicomplexan microneme proteins. The fusion of the surface antigen SAG1 with these transmembrane domains excluded any significant role for the ectodomain in the cleavage site recognition and provided evidence that MPP1 is constitutively active at the surface of the parasites, ready to sustain invasion at any time.

References

Oct 1, 1988·Biomedical & Environmental Mass Spectrometry·K Biemann
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·M CitronD J Selkoe
Feb 23, 1999·Molecular Microbiology·V B Carruthers, L D Sibley
Nov 24, 1999·The EMBO Journal·J T DessensR E Sinden
Mar 16, 2000·Molecular and Biochemical Parasitology·J L LovettL D Sibley
Jul 27, 2000·Experimental Parasitology·J BerthonneauJ L Jacquemin
Oct 29, 2000·The Journal of Biological Chemistry·S BrechtD Soldati
Feb 24, 2001·Cellular Microbiology·V B CarruthersL D Sibley
Sep 21, 2001·The Journal of Biological Chemistry·S A MillerK Kim

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Citations

Apr 9, 2005·Current Genetics·Andrea V Robold, Adrienne R Hardham
Mar 24, 2004·Current Opinion in Cell Biology·Dominique Soldati, Markus Meissner
Mar 5, 2003·Molecular and Biochemical Parasitology·Purnima BhanotCathrine Persson
Jul 26, 2002·Molecular Microbiology·Corinna Opitz, Dominique Soldati
Apr 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Fabiola ParussiniGary E Ward
Jun 19, 2008·Genes & Development·Robert B Rawson
Dec 6, 2006·Antimicrobial Agents and Chemotherapy·Chin Fen TeoVern B Carruthers
Sep 19, 2006·Eukaryotic Cell·Susannah D BrydgesVern B Carruthers
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Stefan H I KappeVictor Nussenzweig
Jul 9, 2008·Annual Review of Genetics·Matthew Freeman
Nov 28, 2007·Annual Review of Pathology·James H McKerrowMohammed Sajid
Dec 3, 2005·PLoS Pathogens·Philippa K HarrisMichael J Blackman
Mar 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Fabien BrossierSinisa Urban
Sep 27, 2006·The Journal of Cell Biology·Rebecca A O'DonnellMichael J Blackman
Dec 17, 2011·Molecular and Biochemical Parasitology·Joana M SantosDominique Soldati-Favre
May 1, 2010·International Journal for Parasitology·Nikolas FriedrichDominique Soldati-Favre
Dec 9, 2008·Seminars in Cell & Developmental Biology·Ya Ha
Nov 22, 2008·Seminars in Cell & Developmental Biology·Matthew Freeman
Sep 2, 2004·Cellular Microbiology·Michael J Blackman
Dec 24, 2004·The EMBO Journal·Marius K LembergMatthew Freeman
Mar 9, 2005·Molecular Microbiology·Vern B Carruthers, Michael J Blackman
Aug 17, 2005·Molecular Microbiology·Steven A HowellMichael J Blackman
Mar 12, 2008·Traffic·Lilach Sheiner, Dominique Soldati-Favre
Oct 20, 2011·Physiologia Plantarum·Ronit Rimon Knopf, Zach Adam
Jun 16, 2010·Molecular Microbiology·Lilach SheinerDominique Soldati-Favre
Oct 5, 2010·The EMBO Journal·Kutti R VinothkumarMatthew Freeman

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