Intramembrane particle aggregation in erythrocyte ghosts. II. The influence of spectrin aggregation
Physicochemical properties of mixtures of spectrin and actin extracted from human erythrocyte ghosts have been correlated with ultrastructural changes observed in freeze-fractured erythrocyte membranes. (1) Extracted mixtures of spectrin and actin have a very low solubility (less than 30 mug/ml) near their isoelectric point, pH 4.8. These mixtures are also precipitated by low concentrations of Ca2+, Mg2+, polylysine or basic proteins. (2) All conditions which precipitate extracts of spectrin and actin also induce aggregation of the intramembrane particles in spectrin-depleted erythrocyte ghosts. Precipitation of the residual spectrin molecules into small patches on the cytoplasmic surface of the ghost membrane is thought to be the cause of particle aggregations, implying an association between the spectrin molecules and the intramembrane particles. (3) When fresh ghosts are exposed to conditions which precipitate extracts of spectrin and actin, only limited particle aggregation occurs. Instead, the contraction of the intact spectrin meshwork induced by the precipitation conditions compresses the lipid bilayer of the membrane, causing it to bleb off particle-free, protein-free vesicles. (4) The absence of protein in these lipid ...Continue Reading
Hapten-sandwich labeling. II. Immunospecific attachment of cell surface markers suitable for scanning electron microscopy
Phase transitions and phase separations in phospholipid membranes induced by changes in temperature, pH, and concentration of bivalent cations
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Membrane ultrastructural changes during calcium phosphate-induced fusion of human erythrocyte ghosts
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Electron microscopic study of reassociation of spectrin and actin with the human erythrocyte membrane
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Molecular defect in the sickle erythrocyte skeleton. Abnormal spectrin binding to sickle inside-our vesicles
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pH-induced denaturation of spectrin changes the interaction of membrane proteins in erythrocyte ghosts. Biochemical and electron microscopic evidence
Rearrangement of intramembranous particles and fusion promoted in chicken erythrocytes by intracellular Ca2+
Exocytosis in vitro: ultrastructure of the isolated sea urchin egg cortex as seen in platinum replicas
Mobilization and aggregation of integral membrane proteins in erythrocytes induced by interaction with influenza virus at acidic pH
Dynamic changes in the distribution of the calcium-activated neutral protease in human red blood cells following cellular insult and altered Ca2+ homeostasis
Effects of bile salts of human erythrocytes. Plasma membrane vesiculation, phospholipid solubilization and their possible relationships to bile secretion
Correlative statistical analysis and computer modelling of intramembraneous particle distributions in human erythrocyte membranes
Intense, reversible aggregation of intramembrane particles in non-haemolyzed human erythrocytes. A freeze-fracture study
Isolation and characterization of large (0.5 - 1.0 micron) cytoskeleton-free vesicles from human and rabbit erythrocytes
Low-pH association of proteins with the membranes of intact red blood cells. II. Studies of the mechanism
Formation of protein polymers in erythrocyte ghosts incubated with sonicated lipid vesicles. Effects on spectrin extractibility, permeability of ghosts to vesicles, intramembrane particle distribution and bleb formation
Studies of water permeability and proteins of erythrocyte membranes in patients with Duchenne muscular dystrophy
Aggregation of myocardial sarcolemmal transmembrane proteins is not hindered by an interaction with the cytoskeleton. Possible implications for ischemia and reperfusion
Low pH-induced hemolysis of erythrocytes is related to the entry of the acid into cytosole and oxidative stress on cellular membranes
Effects of incorporated trypsin on anion exchange and membrane proteins in human red blood cell ghosts
Influence of the intracellular and extracellular cation concentration on monovalent cation efflux of resealed human erythrocyte ghosts
Stabilization of O-pyromellitylgramicidin channels in bilayer lipid membranes through electrostatic interaction with polylysines of different chain lengths
Spin-label studies of the oligomeric structure of band 3 protein in erythrocyte membranes and in reconstituted systems
Induction of ATP depletion, intramembrane particle aggregation and exposure of membrane phospholipids in chicken erythrocytes by local anesthetics and tranquilizers
Vesicle release from rat red cell ghosts and increased association of cell membrane proteins with cytoskeletons induced by cadmium
ESR studies of the erythrocyte membrane skeletal protein network: influence of the state of aggregation of spectrin on the physical state of membrane proteins, bilayer lipids, and cell surface carbohydrates
The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles
Evaluation of IgG molecules, Fab' fragments and IgG-horseradish peroxidase conjugates as surface labels for freeze-etched membranes
Spontaneous, reversible protein cross-linking in the human erythrocyte membrane. Temperature and pH dependence
Influence of temperature and cholesterol on the rotational diffusion of band 3 in the human erythrocyte membrane
Membrane changes in polymorphonuclear leukocytes during ionophore (A23187)-induced lysosomal release
Quantitative composition and characterization of the proteins in membrane vesicles released from erythrocytes by dimyristoylphosphatidylcholine. A membrane system without cytoskeleton
Correlation between distribution of cytoskeletal proteins and release of alkaline phosphatase-rich vesicles by epiphyseal chondrocytes in primary culture
Intraerythrocytic development of Plasmodium knowlesi: structure, temperature- and Ca2+-response of the host and parasite membranes
Ultrastructural study of cholestasis induced by longterm treatment with estradiol valerate. II. Gap junctional analysis
Changes in the functional state of the erythrocyte membrane: significance for red cell filterability and blood viscosity
Quantitative analysis of intramembrane particle (IMP) distribution on biomembranes after freeze-fracture preparation by a computer-based technique
Drug-induced changes in the plasma membrane of Microsporum canis. A preliminary freeze--fracture study
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