Intramitochondrial accumulation of cationic Atto520-biotin proceeds via voltage-dependent slow permeation through lipid membrane

Biochimica Et Biophysica Acta
Yuri N AntonenkoDmitry B Zorov

Abstract

Conjugation to penetrating cations is a general approach for intramitochondrial delivery of physiologically active compounds, supported by a high membrane potential of mitochondria having negative sign on the matrix side. By using fluorescence correlation spectroscopy, we found here that Atto520-biotin, a conjugate of a fluorescent cationic rhodamine-based dye with the membrane-impermeable vitamin biotin, accumulated in energized mitochondria in contrast to biotin-rhodamine 110. The energy-dependent uptake of Atto520-biotin by mitochondria, being slower than that of the conventional mitochondrial dye tetramethyl-rhodamine ethyl ester, was enhanced by the hydrophobic anion tetraphenylborate (TPB). Atto520-biotin also exhibited accumulation in liposomes driven by membrane potential resulting from potassium ion gradient in the presence valinomycin. The induction of electrical current across planar bilayer lipid membrane by Atto520-biotin proved the ability of the compound to permeate through lipid membrane in a cationic form. Atto520-biotin stained mitochondria in a culture of L929 cells, and the staining was enhanced in the presence of TPB. Therefore, the fluorescent Atto520 moiety can serve as a vehicle for intramitochondrial de...Continue Reading

References

Feb 1, 1977·The Journal of General Physiology·E MelnikD C Tosteson
Dec 1, 1989·Biophysical Journal·D L FarkasL M Loew
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Aug 4, 1970·Biochimica Et Biophysica Acta·E A Liberman, V P Skulachev
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·T SanoC R Cantor
May 15, 2004·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·U Ziegler, P Groscurth
Dec 2, 2004·Journal of Molecular Biology·Joseph RosenbluhAbraham Loyter
Jul 14, 2005·Annual Review of Nutrition·Janos Zempleni
Oct 4, 2006·Annual Review of Pharmacology and Toxicology·Michael P Murphy, Robin A J Smith
Feb 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Peter HebbelnThomas Eitinger
Sep 23, 2008·The Journal of Membrane Biology·Tatyana I RokitskayaYuri N Antonenko
Jan 23, 2009·Biochimica Et Biophysica Acta·Vladimir P SkulachevDmitry B Zorov
Apr 2, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Sebastian van de LindeMarkus Sauer
Aug 21, 2009·Bioorganic & Medicinal Chemistry·Ethel J NgenYoungjae You
Oct 20, 2009·Biochimica Et Biophysica Acta·Lucia BiasuttoCristina Paradisi
Feb 1, 2011·Current Drug Targets·Fernanda M CunhaAlicia J Kowaltowski
Apr 2, 2011·The Journal of Biological Chemistry·Yuri N AntonenkoVladimir P Skulachev
Oct 29, 2014·Chemical Communications : Chem Comm·Stepan S DenisovYuri N Antonenko

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.