Intramolecular complementing mutations in tobacco mosaic virus movement protein confirm a role for microtubule association in viral RNA transport

Journal of Virology
V BoykoManfred Heinlein

Abstract

The movement protein (MP) of Tobacco mosaic virus (TMV) facilitates the cell-to-cell transport of the viral RNA genome through plasmodesmata (Pd). A previous report described the functional reversion of a dysfunctional mutation in MP (Pro81Ser) by two additional amino acid substitution mutations (Thr104Ile and Arg167Lys). To further explore the mechanism underlying this intramolecular complementation event, the mutations were introduced into a virus derivative expressing the MP as a fusion to green fluorescent protein (GFP). Microscopic analysis of infected protoplasts and of infection sites in leaves of MP-transgenic Nicotiana benthamiana indicates that MP(P81S)-GFP and MP(P81S;T104I;R167K)-GFP differ in subcellular distribution. MP(P81S)-GFP lacks specific sites of accumulation in protoplasts and, in epidermal cells, exclusively localizes to Pd. MP(P81S;T104I;R167K)-GFP, in contrast, in addition localizes to inclusion bodies and microtubules and thus exhibits a subcellular localization pattern that is similar, if not identical, to the pattern reported for wild-type MP-GFP. Since accumulation of MP to inclusion bodies is not required for function, these observations confirm a role for microtubules in TMV RNA cell-to-cell trans...Continue Reading

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Citations

Dec 3, 2010·Protoplasma·Annette Niehl, Manfred Heinlein
Nov 13, 2003·Current Opinion in Plant Biology·Biao DingYijun Qi
Aug 16, 2002·Current Opinion in Plant Biology·Andrew MauleCarsten Lederer
Oct 24, 2002·Current Opinion in Plant Biology·Manfred Heinlein
Oct 3, 2012·Plant Physiology·Annette NiehlManfred Heinlein
Apr 5, 2008·PLoS Pathogens·Hannes VoglerManfred Heinlein
Jun 16, 2006·Journal of Virology·Mark SeemanpillaiManfred Heinlein
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Daniël Van DammeDanny Geelen
Jun 23, 2007·The Plant Journal : for Cell and Molecular Biology·Vitaly BoykoManfred Heinlein
Feb 6, 2013·The Plant Journal : for Cell and Molecular Biology·Annette NiehlManfred Heinlein
Mar 17, 2010·The Plant Journal : for Cell and Molecular Biology·Maurice O OukoPeter Nick
Jan 20, 2007·Biochemical Society Transactions·C HofmannM Heinlein
Oct 25, 2020·The Plant Journal : for Cell and Molecular Biology·Eduardo J PeñaManfred Heinlein

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