PMID: 3768320Aug 26, 1986Paper

Intramolecular distance measurements in alpha-lactalbumin

Biochemistry
G Musci, L J Berliner

Abstract

The distance between the calcium site (site I) and the zinc site (site II) in alpha-lactalbumin was estimated from Forster energy-transfer measurements between donor Eu(III) [or Tb(III)] at site I and acceptor Co(II) at site II to be 11.5 +/- 1.5 A. Intersite distances were also measured between the bis-ANS [4,4'-bis[1-(phenylamino)-8-naphthalenesulfonate]] binding locus and cobalt at site II (13.6 +/- 1.0 A), between bis-ANS and a fluorescein moiety covalently bound to Met-90 (33.5 +/- 3.0 A), and between Met-90 (fluorescein) and cobalt at site II (16.7 +/- 1.0 A). The apparent Kd for cobalt binding to site II agreed well with the value measured previously by intrinsic fluorescence [Murakami, K., & Berliner, L. J. (1983) Biochemistry 22, 3370-3374]. A Zn(II) titration of Eu(III)-alpha-lactalbumin reconfirmed that both sites I and II can be occupied simultaneously [Musci, G., & Berliner, L. J. (1985) Biochemistry 24, 3852-3856], since the lanthanide fluorescence was unaffected.

References

Apr 1, 1986·Journal of Inorganic Biochemistry·G MusciL J Berliner
Apr 9, 1974·Biochemistry·B W Matthews, L H Weaver
Oct 26, 1982·Biochemistry·K MurakamiL J Berliner
Apr 1, 1984·Molecular and Cellular Biochemistry·L J BerlinerJ E Bell
Aug 1, 1984·Analytical Biochemistry·L Lindahl, H J Vogel

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Citations

Mar 26, 1998·Protein Science : a Publication of the Protein Society·M Narayan, L J Berliner
Oct 1, 1993·Journal of Protein Chemistry·E A PermyakovL J Berliner
Dec 1, 1991·Journal of Protein Chemistry·E A PermyakovL J Berliner
Apr 1, 1994·Journal of Protein Chemistry·E A Permyakov, L J Berliner
Mar 7, 1996·Biochimica Et Biophysica Acta·J M AraminiH J Vogel
Oct 1, 1988·Biophysical Chemistry·E A Permyakov Derezhkov VYu
Dec 26, 2001·Journal of Inorganic Biochemistry·Katrien Noyelle, Herman Van Dael
Dec 1, 1996·Critical Reviews in Food Science and Nutrition·D W WongA E Pavlath
Jun 15, 2006·Antioxidants & Redox Signaling·Lars I Leichert, Ursula Jakob
Dec 1, 1987·Photochemistry and Photobiology·P B O'Hara
Jul 16, 2005·International Journal of Biological Macromolecules·M R HousaindokhtA A Moosavi-Movahedi
Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·M J Kronman
Mar 1, 1996·International Journal of Peptide and Protein Research·N Tanaka, S Kunugi
Dec 11, 2007·Bioconjugate Chemistry·Yihui ChenKoji Nakanishi
Jul 5, 1989·Journal of Molecular Biology·K R AcharyaD C Phillips

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