PMID: 7024086Jun 1, 1981Paper

Intramolecular enzymatic peptide synthesis: trypsin-mediated coupling of the peptide bond between B22-arginine and B23-glycine in a split crosslinked insulin

Hoppe-Seyler's Zeitschrift für physiologische Chemie
S C ChuD Brandenburg

Abstract

The resealing of a split peptide bond (B22/23) in a crosslinked insulin derivative by a trypsin-mediated coupling reaction in Tris buffer/glycerol/dimethylsulphoxide 1 : 1 : 1 at pH 6.5 is described. The coupling yield obtained with N alpha A1, N epsilon B29-(carbonyl-bis-methionyl) insulin [OC(Met)2] was 55-60%, and reproducible yields of OC(Met)2-insulin were 40%. After removing the crosslink and purification, crystalline resynthesized insulin of high biological activity was obtained in a yield of 43% and an overall yield, based on split OC(Met)2-insulin, of 18%.

References

Jan 1, 1977·Advances in Experimental Medicine and Biology·D BrandenburgA Wollmer
Nov 1, 1973·Biochemical and Biophysical Research Communications·R Geiger, R Obermeier
Jun 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·D Brandenburg, A Wollmer
Jan 1, 1974·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A J MoodyJ Gliemann
Jun 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·D Brandenburg
Jun 1, 1981·Hoppe-Seyler's Zeitschrift für physiologische Chemie·C C WangD Brandenburg
Jan 29, 1980·Biochemical and Biophysical Research Communications·K MoriharaT Kanaya
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H TagerE T Kaiser

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Citations

Sep 1, 1982·Applied Biochemistry and Biotechnology·I M ChaikenF Widmer
Feb 15, 1996·Enzyme and Microbial Technology·I GillE N Vulfson

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