Intramolecular fluorescence correlation spectroscopy in a feedback tracking microscope.

Biophysical Journal
Kevin McHale, Hideo Mabuchi

Abstract

We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system and derive a general expression for the fluorescence autocorrelation function that applies when those dynamics are linear. We show that in comparison to traditional fluorescence correlation spectroscopy, tracking provides enhanced sensitivity to translational diffusion, molecular size, heterogeneity, and long-timescale decays. We demonstrate our approach using a three-dimensional tracking microscope to study genomic lambda-phage DNA molecules with various fluorescence label configurations.

References

Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S MaitiW W Webb
Aug 23, 2002·Physical Review Letters·Andrew J BerglundHideo Mabuchi
Sep 25, 2003·Biochemical Society Transactions·V LeviE Gratton
Mar 6, 2004·Physical Review Letters·Roman ShustermanOleg Krichevsky
May 23, 2006·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·R G WinklerJ O Rädler
Feb 7, 2007·Physical Review Letters·E P PetrovP Schwille
Feb 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel NettelsBenjamin Schuler
May 16, 2007·Physical Review Letters·Adam E Cohen, W E Moerner
Oct 24, 2007·Nano Letters·Kevin McHaleHideo Mabuchi
Oct 3, 2005·Optics Express·Andrew Berglund, Hideo Mabuchi
Jun 11, 2007·Optics Express·Andrew J BerglundHideo Mabuchi
Nov 17, 2009·Journal of the American Chemical Society·Kevin McHale, Hideo Mabuchi

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.