PMID: 8598283Mar 1, 1996Paper

Intramolecular inhibition of activating transcription factor-2 function by its DNA-binding domain

Genes & Development
X Y Li, M R Green

Abstract

ATF-2 is a cellular basic region-leucine zipper (bZIP) transcription factor that can mediate diverse transcriptional responses, including activation by the adenovirus Ela protein. ATF-2 contains an activation domain, required for transcriptional activity, but in the absence of an appropriate inducer, full-length ATF-2 is transcriptionally inactive. Here we have investigated the mechanism underlying this regulated inhibition of ATF-2 transcriptional activity. We show that the region of ATF-2 that suppresses the activation region is the bZIP DNA-binding domain and that maximal inhibition requires both the basic region and leucine zipper subdomains. Inhibition is activation domain specific: The ATF-2 bZIP suppresses the ATF-2 and the related Ela activation domains but not acidic- and glutamine-rich activation domains. In vitro protein interaction assays demonstrate that the ATF-2 activation domain and bZIP specifically bind to one another. Finally, we show that bZIP-mediated inhibition can be modulated in a cell-type-specific manner by another sequence element within ATF-2. On the basis of these and other data, we propose that the ATF-2 bZIP and activation domain are engaged in an inhibitory intramolecular interaction and that ind...Continue Reading

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Citations

Nov 28, 2002·Biochimie·Ahmet CivasSébastien Navarro
Dec 24, 2009·Nature Reviews. Cancer·Pablo Lopez-BergamiZe'ev Ronai
Dec 6, 2002·Nucleic Acids Research·Pierre-Antoine Defossez, Eric Gilson
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Nov 22, 2007·PloS One·Bernhard G BaumgartnerAntonio Zorzano
Mar 7, 2014·PloS One·Ting-Yu LinLi-Kwan Chang
Feb 19, 2013·Oncogene·J WalczynskiW Breitwieser
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·H WatadaM S German
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Oct 16, 2007·Pigment Cell Research·Anindita BhoumikZe'ev Ronai
Oct 13, 2011·Head & Neck·Dianne DuffeyGoutham Krishna Gorti
Jan 21, 2012·Biochemical Society Transactions·Malgorzata Gozdecka, Wolfgang Breitwieser
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Sep 1, 2000·Journal of Molecular Biology·M A van TilborgR Kaptein
Oct 24, 1998·The Journal of Biological Chemistry·Y SanoS Ishii

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