PMID: 785236Jan 1, 1976

Intramolecular mobility of pepsin

Molekuliarnaia biologiia
B O GlotovL L Zavada

Abstract

By modifying four tyrosine residues in pepsin a derivative (aminopepsin) is obtained which is capable to conjugate with a fluorescent label DNS-Cl without loss of the catalytic activity. Rotational relaxation times of native pepsin and dansylated aminopepsin (DAP) are measured by a fluoresence polarization method. The values obtained are shown to be lower than those calculated for arigid pepsin globule. Possible sources of the obtained difference are discussed. A reversible or covalent blocking of pepsin or DAP active centres by specific inhibitors leads to an increase in rotational relaxation time values for these proteins reaching the magnitude which is very close to that calculated for a model of rigid pepsin. Brownian relaxation of pepsin and DAP reduced by beta-mercaptoethanol and of pepsinogen and some fragments of pepsine macromolecule in aqueous solutions is invigated as well. The results are intepreted as representing an intramolecular mobility or segmental flexibility of pepsin and DAP. With the use of the obtained and X-ray data a segmental model of dynamic pepsin structure is suggested. On the basis of this model some conclusions are drawn concerning a localization of a polypeptide which is split off from the N-term...Continue Reading

Related Concepts

Catalysis
2-Mercaptoethanol
Pepsin 3

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