Intramural multisite recording of transmembrane potential in the heart

Biophysical Journal
Darren A HooksBruce H Smaill

Abstract

Heart surface optical mapping of transmembrane potentials has been widely used in studies of normal and pathological heart rhythms and defibrillation. In these studies, three-dimensional spatio-temporal events can only be inferred from two-dimensional surface potential maps. We present a novel optical system that enables high fidelity transmural recording of transmembrane potentials. A probe constructed from optical fibers is used to deliver excitation light and collect fluorescence from seven positions, each 1 mm apart, through the left ventricle wall of the rabbit heart. Excitation is provided by the 488-nm line of a water-cooled argon-ion laser. The fluorescence of the voltage-sensitive dye di-4-ANEPPS from each tissue site is split at 600 nm and imaged onto separate photodiodes for later signal ratioing. The optics and electronics are easily expandable to accommodate multiple optical probes. The system is used to record the first simultaneous measurements of transmembrane potential at a number of sites through the intact heart wall.

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Citations

Oct 12, 2012·Pflügers Archiv : European journal of physiology·Peter LeeChristian Bollensdorff
Jan 22, 2005·Clinical and Experimental Pharmacology & Physiology·Bruce H SmaillPeter J Hunter
Apr 10, 2003·Journal of Biomedical Optics·Venkat K Ramshesh, Stephen B Knisley
Jul 7, 2007·Journal of Biomedical Optics·Dean C S TaiChristian Soeller
Feb 22, 2012·Circulation Research·Todd J HerronJosé Jalife
Mar 5, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Emilia Entcheva
Sep 8, 2010·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Richard D WaltonOlivier Bernus
Dec 18, 2003·Journal of Cardiovascular Electrophysiology·Jonathan L ByarsVladimir G Fast
Dec 18, 2003·Journal of Cardiovascular Electrophysiology·André G Kleber
Feb 14, 2006·Experimental Physiology·Natalia Trayanova
Oct 26, 2013·IEEE Reviews in Biomedical Engineering·Bas J Boukens, Igor R Efimov
Jul 14, 2004·IEEE Transactions on Bio-medical Engineering·Matthew W KayJack M Rogers
Feb 14, 2008·IEEE Transactions on Bio-medical Engineering·Darren A Hooks, Mark L Trew
Aug 19, 2006·IEEE Transactions on Bio-medical Engineering·Salim F Idriss, Ann M Pitruzzello
Apr 27, 2005·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Deborah Langrill Beaudoin, Bradley J Roth
Sep 15, 2011·IEEE Transactions on Bio-medical Engineering·Wei KongVladimir G Fast
Oct 3, 2003·Journal of Cardiovascular Electrophysiology·Matthew W Kay, Jack M Rogers
Dec 28, 2010·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Jean ProvostElisa E Konofagou
Sep 21, 2004·Biophysical Journal·Jenny R HolzerFranz Baudenbacher
Jul 28, 2016·Biophysical Journal·Hanyu ZhangJack M Rogers
Apr 21, 2007·Heart, Lung & Circulation·Loraine K Holley
Aug 24, 2002·Circulation Research·Darren A HooksPeter J Hunter
Jul 10, 2004·Circulation Research·Igor R EfimovGuy Salama
Feb 13, 2019·Cardiovascular Research·Peter LeeDavid Filgueiras-Rama
Sep 22, 2005·Journal of Cardiovascular Electrophysiology·Bryan J CaldwellBruce H Smaill
Nov 30, 2006·Annals of the New York Academy of Sciences·Travis M AustinMark L Trew
Nov 12, 2014·Annals of Biomedical Engineering·Prashanna KhwaounjooBruce H Smaill
May 8, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Dean C-S TaiBruce H Smaill
Aug 15, 2014·Journal of Biomedical Optics·Aleksandra Klimas, Emilia Entcheva
May 15, 2004·Progress in Biophysics and Molecular Biology·P J Hunter

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