Intramuscular delivery of mIL-12 gene reduces the expression of CD44/CD49d on pulmonary leucocytes and inhibits ovalbumin-induced airway hyperreactivity

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
H LiW Chen

Abstract

To investigate the effects of murine IL-12 (mIL-12) gene on the expression of CD44 and CD49d on pulmonary leukocytes. BALB/c mice (n = 24) were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged with OVA aerosol for 7 consecutive days. mIL-12 plasmid was then intramuscularly administered in eight BALB/c mice every second day for three times during OVA challenge. Administration of mIL-12 plasmid significantly reduced airway hyperreactivity, the expression of CD44 and CD49d on pulmonary leukocytes, pulmonary infiltration of inflammatory cells, Th2 cytokines production and goblet cell hyperplasia (P < 0.05). Importantly, the percentages of CD44+/CD49d+ leucocytes showed positive correlations with numbers of inflammatory cells, goblet cells hyperplasia and levels of IL-4 and IL-5 (P < 0.01). Our study suggests that CD44 and CD49d might contribute to the preventive effects of mIL-12 gene in asthma.

Citations

Feb 16, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Qing WangJianying Zhou
Jan 19, 2011·Current Allergy and Asthma Reports·Tania MaesGuy F Joos
Sep 22, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Yinan YaoJianying Zhou
Dec 9, 2016·Human Gene Therapy·Dolan SondhiRonald G Crystal
Jun 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qing WangJianying Zhou
Dec 17, 2008·Current Opinion in Pulmonary Medicine

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