Intranasal administration of poly(I:C) and LPS in BALB/c mice induces airway hyperresponsiveness and inflammation via different pathways.

PloS One
Magnus StarkhammarLars Olaf Cardell

Abstract

Bacterial and viral infections are known to promote airway hyperresponsiveness (AHR) in asthmatic patients. The mechanism behind this reaction is poorly understood, but pattern recognizing Toll-like receptors (TLRs) have recently been suggested to play a role. To explore the relation between infection-induced airway inflammation and the development of AHR, poly(I:C) activating TLR3 and LPS triggering TLR4, were chosen to represent viral and bacterial induced interactions, respectively. Female BALB/c or MyD88-deficient C57BL/6 mice were treated intranasally with either poly(I:C), LPS or PBS (vehicle for the control group), once a day, during 4 consecutive days. When methacholine challenge was performed on day 5, BALB/c mice responded with an increase in airway resistance. The maximal resistance was higher in the poly(I:C) and LPS treated groups than among the controls, indicating development of AHR in response to repeated TLR activation. The proportion of lymphocytes in broncheoalveolar lavage fluid (BALF) increased after poly(I:C) treatment whereas LPS enhanced the amount of neutrophils. A similar cellular pattern was seen in lung tissue. Analysis of 21 inflammatory mediators in BALF revealed that the TLR response was receptor-...Continue Reading

References

Jul 7, 1999·Lancet·I D PavordA J Wardlaw
Nov 11, 1999·American Journal of Respiratory and Critical Care Medicine·T J HuangK F Chung
Apr 1, 2000·Australian Family Physician·J Burdon
Aug 5, 2000·Allergy·E MicilloG F Abbate
Feb 15, 2001·The New England Journal of Medicine·W W Busse, R F Lemanske
Sep 20, 2002·The New England Journal of Medicine·Charlotte Braun-FahrländerUNKNOWN Allergy and Endotoxin Study Team
Mar 10, 2004·Chest·Gustavo J RodrigoJesse B Hall
Aug 10, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M BrybornL-O Cardell
Sep 9, 2005·Respiratory Research·Mattias FranssonLars-Olaf Cardell
Apr 4, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiaoyin ShanMichael M Grunstein
May 9, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ofir BacharLars-Olaf Cardell
Jul 1, 2006·Journal of Leukocyte Biology·Dieudonnée TogbeValerie F J Quesniaux
Jul 15, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Z I KomlósiG Losonczy
Jul 1, 2008·Current Allergy and Asthma Reports·Omar Tliba, Reynold A Panettieri
Jul 10, 2008·Respiratory Physiology & Neurobiology·E M BozanichP D Sly
Nov 19, 2008·The Journal of Allergy and Clinical Immunology·Annemarie Sykes, Sebastian L Johnston
Nov 26, 2008·Paediatric Respiratory Reviews·Jodie L SimpsonJeroen Douwes
Jan 8, 2009·International Archives of Allergy and Immunology·Anna-Karin EkmanLars-Olaf Cardell
Jan 9, 2009·Journal of Leukocyte Biology·Anne Månsson, Lars-Olaf Cardell
Apr 24, 2009·Proceedings of the American Thoracic Society·Dawn C Newcomb, R Stokes Peebles
May 21, 2010·International Archives of Allergy and Immunology·Linda SwedinMikael Adner
Jul 14, 2010·Trends in Immunology·Charlotte SummersEdwin R Chilvers
Jan 25, 2011·Pulmonary Pharmacology & Therapeutics·Jesper SäfholmMikael Adner

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Citations

Mar 29, 2014·American Journal of Respiratory Cell and Molecular Biology·Yuan XuLars-Olaf Cardell
Feb 19, 2013·Expert Opinion on Therapeutic Targets·Tsukasa SeyaMisako Matsumoto
Feb 29, 2016·Respirology : Official Journal of the Asian Pacific Society of Respirology·Rakesh K KumarPaul S Foster
Jan 1, 2014·Journal of the American Chemical Society·Roy WeinstainRoger Y Tsien
Sep 14, 2015·Neuroscience and Biobehavioral Reviews·David Chun-Hei PoonRaymond Chuen-Chung Chang
Jul 7, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Valentina Moyano-PorcileMarcela Julio-Pieper
Jun 11, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Jutta RenkonenRisto Renkonen
Mar 31, 2015·PloS One·Chantal DonovanJane E Bourke
Aug 3, 2016·Regenerative Biomaterials·Lei RongXian-Zheng Zhang
Apr 13, 2017·Respirology : Official Journal of the Asian Pacific Society of Respirology·Steven MaltbyPaul S Foster
May 27, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yuan Xu, Lars-Olaf Cardell
Aug 1, 2018·Annals of the New York Academy of Sciences·Jamela JoudaAdriana Del Rey
Aug 19, 2017·Journal of Extracellular Vesicles·Rosario MarotoAllan R Brasier
Oct 4, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Sara HadjigolPaul S Foster
Apr 3, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thi Hiep NguyenMing Yang
Jan 31, 2018·Frontiers in Immunology·Leandro do Nascimento CamargoIolanda de Fátima Lopes Calvo Tibério
Jun 7, 2014·Journal of Leukocyte Biology·Rakesh K KumarHelene F Rosenberg
Sep 28, 2018·Current Allergy and Asthma Reports·Kathryn R MichelsWendy Fonseca
Oct 6, 2020·Frontiers in Pharmacology·Leandro do Nascimento CamargoIolanda de Fátima Lopes Calvo Tibério

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Methods Mentioned

BETA
lavage

Software Mentioned

Graph Pad Prism
GraphPad

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