Intranasal and intramuscular immunization with Baculovirus Dual Expression System-based Pvs25 vaccine substantially blocks Plasmodium vivax transmission

Vaccine
Andrew M BlagboroughR E Sinden

Abstract

We have recently developed a new experimental vaccine vector system based on Autographa californica nucleopolyhedrosis virus (AcNPV) termed the "Baculovirus Dual Expression System", which drives expression of vaccine candidate antigens by a dual promoter that consists of tandemly arranged baculovirus-derived polyhedrin and mammalian-derived CMV promoters. The present study used this system to generate a Plasmodium vivax transmission-blocking immunogen (AcNPV-Dual-Pvs25). AcNPV-Dual-Pvs25 not only displayed Pvs25 on the AcNPV envelope, exhibiting aspects of its native three-dimensional structure, but also expressed appropriately immunogenic protein upon transduction of mammalian cells. Both intranasal and intramuscular immunization of mice with AcNPV-Dual-Pvs25 induced high Pvs25-specific antibody titres, notably of IgG1, IgG2a and IgG2b isotypes, indicating a mixed Th1/Th2 response. Importantly, sera obtained from subcutaneously immunized rabbits exhibited a significant transmission-blocking effect (96% reduction in infection intensity, 24% reduction in prevalence) when challenged with human blood infected with P. vivax gametocytes using the standard membrane feeding assay. Additionally, active immunization (both intranasal and...Continue Reading

References

Nov 1, 1992·Trends in Biotechnology·D C KaslowP J Barr
Oct 1, 1995·Bio/technology·A H Davies
Mar 21, 2001·Vaccine·R Carter
Dec 1, 2001·Expert Opinion on Biological Therapy·A Stowers, R Carter
Nov 13, 2002·Cellular Microbiology·R E Sinden
Sep 8, 2004·Journal of Immunological Methods·Kota Arun KumarVictor Nussenzweig

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Citations

Aug 4, 2011·Journal of Invertebrate Pathology·António RoldãoPaula M Alves
Sep 2, 2015·Vaccine·Stephen L HoffmanPatrick E Duffy
May 21, 2017·Expert Review of Vaccines·Ahmad Syibli OthmanShahid M Khan
Oct 27, 2017·Parasite Immunology·M IyoriS Yoshida
Dec 17, 2019·Immunological Reviews·Roos M de JongTeun Bousema
May 12, 2020·Journal of Leukocyte Biology·Mario Fragoso-Saavedra, Marco A Vega-López
Sep 14, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Talha Bin EmranShigeto Yoshida
Aug 27, 2014·Expert Review of Vaccines·Shih-Yeh LinYu-Chen Hu
Jun 3, 2018·Viruses·Balraj PremanandMookkan Prabakaran
Jan 23, 2021·Expert Review of Vaccines·Patrick E Duffy

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